ArticleExperimental animals2018
Reproducible insulin secretion from isolated rat pancreas preparations using an organ bath.
Article in Experimental animals, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 6 citations in OpenAlex.
- Development of an organ bath technique for isolated rat pancreas preparations to assess the effect of 1,5-AG on insulin secretion.Experimental animals · 2020Article
- MARCKS phosphorylation and amylase release in GLP-1-stimulated acini isolated from rat pancreas.The journal of physiological sciences : JPS · 2019Article
- The Effects of Trypsin Inhibitor on Insulin Secretion Using Rat Pancreas in an Organ Bath.In vivo (Athens, Greece)Article
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Authors and funding
11 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Diabetes mellitus is a lifestyle-related disease that is characterized by inappropriate or diminished insulin secretion. Ex vivo pharmacological studies of hypoglycemic agents are often conducted using perfused pancreatic preparations. Pancreas preparations for organ bath experiments do not require cannulation and are therefore less complex than isolated perfused pancreas preparations. However, previous research has generated almost no data on insulin secretion from pancreas preparations using organ bath preparations. The purpose of this study was to investigate the applicability of isolated rat pancreas preparations using the organ bath technique in the quantitative analysis of insulin secretion from β-cells. We found that insulin secretion significantly declined during incubation in the organ bath, whereas it was maintained in the presence of 1 µM GLP-1. Conversely, amylase secretion exhibited a modest increase during incubation and was not altered in the presence of GLP-1. These results demonstrate that the pancreatic organ bath preparation is a sensitive and reproducible method for the ex vivo assessment of the pharmacological properties of hypoglycemic agents.
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