ArticleScientific reports2017
A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.
- Chronotype and disordered eating indicators: A systematic review and meta-analysis.Clinical psychology review · 2026Pooled it
- Low temperature abolishes human cellular circadian rhythm through Hopf bifurcation.NPJ systems biology and applications · 2025Article
- Mapping cell dynamics in human ex vivo hair follicles suggests pulling mechanism of hair growth.Nature communications · 2025Article
- Circadian Oscillations in Skin and Their Interconnection with the Cycle of Life.International journal of molecular sciences · 2023Review
- Analysis of the Anticipatory Behavior Formation Mechanism Induced by Methamphetamine Using a Single Hair.Cells · 2023Article
- Hair Follicles as a Critical Model for Monitoring the Circadian Clock.International journal of molecular sciences · 2023Review
- An efficient, non-invasive approach forAging · 2021Article
- The Impact of the Circadian Clock on Skin Physiology and Cancer Development.International journal of molecular sciences · 2021Review
- Article
- The molecular clock in the skin, its functionality, and how it is disrupted in cutaneous melanoma: a new pharmacological target?Cellular and molecular life sciences : CMLS · 2019Review
- Article
- A Method for Culturing Mouse Whisker Follicles to Study Circadian RhythmsBio-protocol · 2019Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Almost all organisms maintain a circadian clock from birth to death to synchronize their own physiology and behavior with the earth's rotation. Because the in vivo evaluation of human circadian characteristics is labor-intensive, in vitro or ex vivo approaches could provide advantages. In this study, to enable the simple and non-invasive evaluation of autonomous circadian oscillation, we established a method for monitoring clock gene expression by performing ex vivo culture of whole hair root tissue. This method is extremely simple and imposes little burden on subjects. Results obtained using Cryptochrome-deficient mice support that circadian period length in hair tissue correlates with intrinsic period length observed in physiology and behavior. We then applied this method to old-old subjects with severe dementia, who showed abnormal circadian behavior, and found that their peripheral clocks autonomously oscillated in a manner similar to those of healthy or younger subjects, indicating that the effect of cellular senescence on the autonomous clock oscillator is limited at least in some cell types. Although further validation may be required, the hair tissue-based culture assay would be a tool to investigate intrinsic circadian characteristics in humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.