ArticleScientific reports2017
ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
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- Development of three-colour FRET cascade for force sensing of the putative RIAM-vinculin interaction in fibroblasts.Communications chemistry · 2025Article
- Multiplexed Dark FRET Biosensors: An accessible live-cell platform for target- and cell-specific monitoring of protein-protein interactions in 2D and 3D model systems.Research square · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
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- A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells.Nature communications · 2023Article
- LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics.Biophysics and physicobiology · 2023Article
- Structure-based analysis and evolution of a monomerized red-colored chromoprotein from the Olindias formosa jellyfish.Protein science : a publication of the Protein Society · 2022Article
- Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging.Nature communications · 2021Article
- Genetically Encoded Fluorescent Biosensors for Biomedical Applications.Biomedicines · 2021Review
- Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor.Nature communications · 2021Article
- Photoactivatable CaMKII induces synaptic plasticity in single synapses.Nature communications · 2021Article
- Optogenetic Imaging of Protein Activity Using Two-Photon Fluorescence Lifetime Imaging Microscopy.Advances in experimental medicine and biology · 2021Review
- Resonance energy transfer sensitises and monitors in situ switching of LOV2-based optogenetic actuators.Nature communications · 2020Article
- Fluorescent Biosensors for Neuronal Metabolism and the Challenges of Quantitation.Current opinion in neurobiology · 2020Review
- Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications.Journal of biomedical optics · 2020Review
- FRET as a biomolecular research tool - understanding its potential while avoiding pitfalls.Nature methods · 2019Review
- ShadowR: a novel chromoprotein with reduced non-specific binding and improved expression in living cells.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence lifetime imaging microscopy (FLIM)-based Förster resonance energy transfer (FRET) measurement (FLIM-FRET) is one of the powerful methods for imaging of intracellular protein activities such as protein-protein interactions and conformational changes. Here, using saturation mutagenesis, we developed a dark yellow fluorescent protein named ShadowY that can serve as an acceptor for FLIM-FRET. ShadowY is spectrally similar to the previously reported dark YFP but has a much smaller quantum yield, greater extinction coefficient, and superior folding property. When ShadowY was paired with mEGFP or a Clover mutant (Clover
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