ArticleJournal of cell science2017
Cells lay their own tracks - optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration.
Article in Journal of cell science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Actin-membrane interface stress regulates Arp2/3-branched actin density during lamellipodial protrusion.bioRxiv : the preprint server for biology · 2026Article
- Closed-loop optogenetic control of cell biology enables outcome-driven microscopy.Nature communications · 2025Article
- The role and regulation of integrins in cell migration and invasion.Nature reviews. Molecular cell biology · 2025Review
- Deposited footprints let cells switch between confined, oscillatory, and exploratory migration.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Differential regulation of fibronectin expression and fibrillogenesis by autocrine TGF-β1 signaling in malignant and benign mammary epithelial cells.The international journal of biochemistry & cell biology · 2023Article
- Fibroblasts secrete fibronectin under lamellipodia in a microtubule- and myosin II-dependent fashion.The Journal of cell biology · 2023Article
- Quantifying protrusions as tumor-specific biophysical predictors of cancer invasion in in vitro tumor micro-spheroid models.In vitro models · 2022Article
- Computational exploration of treadmilling and protrusion growth observed in fire ant rafts.PLoS computational biology · 2022Article
- The principles of directed cell migration.Nature reviews. Molecular cell biology · 2021Review
- A functional antagonism between RhoJ and Cdc42 regulates fibronectin remodelling during angiogenesis.Small GTPases · 2021Article
- The fluid shear stress sensor TRPM7 regulates tumor cell intravasation.Science advances · 2021Article
- Optogenetics: The Art of Illuminating Complex Signaling Pathways.Physiology (Bethesda, Md.) · 2021Review
- Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins.Small GTPases · 2020Article
- A Weak Link with Actin Organizes Tight Junctions to Control Epithelial Permeability.Developmental cell · 2020Article
- Coronin 1C inhibits melanoma metastasis through regulation of MT1-MMP-containing extracellular vesicle secretion.Scientific reports · 2020Article
- Article
- Designing protein structures and complexes with the molecular modeling program Rosetta.The Journal of biological chemistry · 2019Review
- Control of adhesion and protrusion in cell migration by Rho GTPases.Current opinion in cell biology · 2019Review
- Perspectives of RAS and RHEB GTPase Signaling Pathways in Regenerating Brain Neurons.International journal of molecular sciences · 2018Review
- Optogenetic dissection of Rac1 and Cdc42 gradient shaping.Nature communications · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Rho GTPase family members are known regulators of directed migration and therefore play key roles in processes including development, the immune response and cancer metastasis. However, their individual contributions to these processes are complex. Here, we modify the activity of the two Rho GTPase family members Rac and Cdc42 by optogenetically recruiting specific guanine nucleotide exchange factor (GEF) DH or PH domains to defined regions of the cell membrane. We find that the localized activation of both GTPases produces lamellipodia in cells plated on a fibronectin substrate. By using a novel optotaxis assay, we show that biased activation can drive directional migration. Interestingly, in the absence of exogenous fibronectin, Rac activation is insufficient to produce stable lamellipodia or directional migration whereas Cdc42 activation is sufficient for these processes. We find that a remarkably small amount of fibronectin (<10 puncta per protrusion) is necessary to support stable GTPase-driven lamellipodia formation. Cdc42 bypasses the need for exogenous fibronectin by stimulating cellular fibronectin deposition under the newly formed lamellipodia.This article has an associated First Person interview with the first author of the paper.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.