ArticlePloS one2017
An increase in immature β-cells lacking Glut2 precedes the expansion of β-cell mass in the pregnant mouse.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- A high-fat, high-sugar diet impairs maternal metabolism throughout pregnancy and lactation in mice.The Journal of physiology · 2026Article
- Naphthoquinones mediate differentiation of human umbilical cord derived mesenchymal stem cells into insulin producing cells through regulation of Wnt and BMP pathways.BioImpacts : BI · 2026Article
- Cafeteria diet compromises natural adaptations of islet cell transdifferentiation and turnover in pregnancy.Diabetic medicine : a journal of the British Diabetic Association · 2025Article
- The Importance of Intra-Islet Communication in the Function and Plasticity of the Islets of Langerhans during Health and Diabetes.International journal of molecular sciences · 2024Review
- Maternal diet during pregnancy and adaptive changes in the maternal and fetal pancreas have implications for future metabolic health.Frontiers in endocrinology · 2024Review
- Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice.Journal of the Endocrine Society · 2023Article
- A highly sensitive strategy for monitoring real-time proliferation of targeted cell types in vivo.Nature communications · 2023Article
- Mouse Models of Gestational Diabetes Mellitus and Its Subtypes: Recent Insights and Pitfalls.International journal of molecular sciences · 2023Review
- Pretransplant HOMA-β Is Predictive of Insulin Independence in 7 Patients With Chronic Pancreatitis Undergoing Islet Autotransplantation.Transplantation direct · 2022Article
- The intestine and the microbiota in maternal glucose homeostasis during pregnancy.The Journal of endocrinology · 2022Review
- Pleiotrophin Expression and Actions in Pancreatic β-Cells.Frontiers in endocrinology · 2022Article
- Article
- Characterisation of Ppy-lineage cells clarifies the functional heterogeneity of pancreatic beta cells in mice.Diabetologia · 2021Article
- Acetone Ingestion Mimics a Fasting State to Improve Glucose Tolerance in a Mouse Model of Gestational Hyperglycemia.International journal of molecular sciences · 2021Article
- Article
- Exposure to Δ9-tetrahydrocannabinol during rat pregnancy leads to impaired cardiac dysfunction in postnatal life.Pediatric research · 2021Article
- Ontology of the apelinergic system in mouse pancreas during pregnancy and relationship with β-cell mass.Scientific reports · 2021Article
- In Utero Exposure to Δ9-Tetrahydrocannabinol Leads to Postnatal Catch-Up Growth and Dysmetabolism in the Adult Rat Liver.International journal of molecular sciences · 2021Article
- Cell Cycle Regulation of the Pdx1 Transcription Factor in Developing Pancreas and Insulin-Producing β-Cells.Diabetes · 2021Article
- Increased alpha and beta cell mass during mouse pregnancy is not dependent on transdifferentiation.Experimental biology and medicine (Maywood, N.J.) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A compensatory increase in β-cell mass occurs during pregnancy to counter the associated insulin resistance, and a failure in adaptation is thought to contribute to gestational diabetes. Insulin-expressing but glucose-transporter-2-low (Ins+Glut2LO) progenitor cells are present in mouse and human pancreas, being predominantly located in extra-islet β-cell clusters, and contribute to the regeneration of the endocrine pancreas following induced ablation. We therefore sought to investigate the contribution of Ins+Glut2LO cells to β-cell mass expansion during pregnancy. Female C57Bl/6 mice were time mated and pancreata were collected at gestational days (GD) 6, 9, 12, 15, and 18, and postpartum D7 (n = 4/time-point) and compared to control (non-pregnant) animals. Beta cell mass, location, proliferation (Ki67+), and proportion of Ins+Glut2LO cells were measured using immunohistochemistry and bright field or confocal microscopy. Beta cell mass tripled by GD18 and β-cell proliferation peaked at GD12 in islets (≥6 β-cells) and small β-cell clusters (1-5 β-cells). The proportion and fraction of Ins+Glut2LO cells undergoing proliferation increased significantly at GD9 in both islets and clusters, preceding the increase in β-cell mass and proliferation, and their proliferation within clusters persisted until GD15. The overall number of clusters increased significantly at GD9. Quantitative PCR showed a significant increase in Pdx1 presence at GD9 vs. GD18 or control pancreas, and Pdx1 was visualized by immunohistochemistry within both Ins+Glut2LO and Ins+Glut2HI cells within clusters. These results indicate that Ins+Glut2LO cells are likely to contribute to β-cell mass expansion during pregnancy.
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