ArticlePLoS neglected tropical diseases2017
Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability.
Article in PLoS neglected tropical diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Interactions Between Inbreeding, Fitness and the Bacterial Microbiome inEvolutionary applications · 2026Article
- Addition of a single bacterial isolate to conventional larval rearing water can impact wing size and longevity in adult malebioRxiv : the preprint server for biology · 2026Article
- Diversity of culturable gut bacteria and their role in conferring resistance to alpha-cypermethrin in field populations ofFrontiers in microbiology · 2026Article
- Transplant of cryopreserved mosquito gut microbiota reveals strain-specific patterns of microbial acquisition and assembly.ISME host microbe · 2026Article
- Article
- Microbiome diversity in mosquitoes and sand flies: implications for vector competence.Parasites & vectors · 2025Review
- Leveraging microbial ecology for mosquito-borne disease control.Trends in parasitology · 2025Review
- Optimizing Aedes albopictus rearing: effects of insect- and bacteria-based larval diets on immature and adult performance.Scientific reports · 2025Article
- Reduced microbe abundance in an urban larval development container increases Aedes aegypti susceptibility to Zika virus.PLoS pathogens · 2025Article
- Thermal variation influences the transcriptome of the major malaria vector Anopheles stephensi.Communications biology · 2025Article
- Mosquitoes Reared in Nearby Insectaries at the Same Institution Have Significantly Divergent Microbiomes.Environmental microbiology · 2025Article
- Mosquitoes reared in distinct insectaries within an institution in close spatial proximity possess significantly divergent microbiomes.bioRxiv : the preprint server for biology · 2024Article
- Article
- The influence of the larval microbiome on susceptibility to Zika virus is mosquito genotype-dependent.PLoS pathogens · 2023Article
- Variable microbiomes between mosquito lines are maintained across different environments.PLoS neglected tropical diseases · 2023Article
- Holobiont perspectives on tripartite interactions among microbiota, mosquitoes, and pathogens.The ISME journal · 2023Review
- Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues.PLoS neglected tropical diseases · 2023Article
- Recovery of metagenomic data from theWellcome open research · 2023Article
- The buzz in the field: the interaction between viruses, mosquitoes, and metabolism.Frontiers in cellular and infection microbiology · 2023Review
- Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes.Microbiome · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The mosquito midgut microbiota has been shown to influence vector competence for multiple human pathogens. The microbiota is highly variable in the field, and the sources of this variability are not well understood, which limits our ability to understand or predict its effects on pathogen transmission. In this work, we report significant variation in female adult midgut bacterial load between strains of A. aegypti which vary in their susceptibility to dengue virus. Composition of the midgut microbiome was similar overall between the strains, with 81-92% of reads coming from the same five bacterial families, though we did detect differences in the presence of some bacterial families including Flavobacteriaceae and Entobacteriaceae. We conducted transcriptomic analysis on the two mosquito strains that showed the greatest difference in bacterial load, and found that they differ in transcript abundance of many genes implicated in amino acid metabolism, in particular the branched chain amino acid degradation pathway. We then silenced this pathway by targeting multiple genes using RNA interference, which resulted in strain-specific bacterial proliferation, thereby eliminating the difference in midgut bacterial load between the strains. This suggests that the branched chain amino acid (BCAA) degradation pathway controls midgut bacterial load, though the mechanism underlying this remains unclear. Overall, our results indicate that amino acid metabolism can act to influence the midgut microbiota. Moreover, they suggest that genetic or physiological variation in BCAA degradation pathway activity may in part explain midgut microbiota variation in the field.
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