ArticleAnalytical chemistry2017
Microscale Mass Spectrometry Analysis of Extracellular Metabolites in Live Multicellular Tumor Spheroids.
Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Developing a Cell Quenching Method to Facilitate Single Cell Mass Spectrometry Metabolomics Studies.JACS Au · 2025Article
- Exploring Single-Probe Single-Cell Mass Spectrometry: Current Trends and Future Directions.Analytical chemistry · 2025Review
- MetaPhenotype: A Transferable Meta-Learning Model for Single-Cell Mass Spectrometry-Based Cell Phenotype Prediction Using Limited Number of Cells.Analytical chemistry · 2024Article
- MassLite: An integrated python platform for single cell mass spectrometry metabolomics data pretreatment with graphical user interface and advanced peak alignment method.Analytica chimica acta · 2024Article
- Three-Dimensional Mass Spectrometry Imaging Reveals Distributions of Lipids and the Drug Metabolite Associated with the Enhanced Growth of Colon Cancer Cell Spheroids Treated with Triclosan.Analytical chemistry · 2022Article
- Single-Cell Mass Spectrometry Enables Insight into Heterogeneity in Infectious Disease.Analytical chemistry · 2022Article
- Lipidomic comparison of 2D and 3D colon cancer cell culture models.Journal of mass spectrometry : JMS · 2022Article
- Single cell mass spectrometry studies reveal metabolomic features and potential mechanisms of drug-resistant cancer cell lines.Analytica chimica acta · 2022Article
- Extract Metabolomic Information from Mass Spectrometry Images Using Advanced Data Analysis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Spatially resolved quantification of drug metabolism and efficacy in 3D paper-based tumor mimics.Analytica chimica acta · 2021Article
- GeenaR: A Web Tool for Reproducible MALDI-TOF Analysis.Frontiers in genetics · 2021Article
- Redesigning the T-probe for mass spectrometry analysis of online lysis of non-adherent single cells.Analytica chimica acta · 2019Article
- Multimodal Imaging of Amyloid Plaques: Fusion of the Single-Probe Mass Spectrometry Image and Fluorescence Microscopy Image.Analytical chemistry · 2019Article
- Quantification of Drug Molecules in Live Single Cells Using the Single-Probe Mass Spectrometry Technique.Analytical chemistry · 2019Article
- Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells.Journal of visualized experiments : JoVE · 2019Article
- Anticancer Drug Affects Metabolomic Profiles in Multicellular Spheroids: Studies Using Mass Spectrometry Imaging Combined with Machine Learning.Analytical chemistry · 2019Article
- Metabolomic Studies of Live Single Cancer Stem Cells Using Mass Spectrometry.Analytical chemistry · 2019Article
- Mass Spectrometry Measurement of Single Suspended Cells Using a Combined Cell Manipulation System and a Single-Probe Device.Analytical chemistry · 2019Article
- On the intersection of electrochemistry and mass spectrometry.Current opinion in electrochemistry · 2019Article
- Towards enhanced metabolomic data analysis of mass spectrometry image: Multivariate Curve Resolution and Machine Learning.Analytica chimica acta · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Extracellular compounds in tumors play critical roles in intercellular communication, tumor proliferation, and cancer cell metastasis. However, the lack of appropriate techniques leads to limited studies of extracellular metabolite. Here, we introduced a microscale collection device, the Micro-funnel, fabricated from biocompatible fused silica capillary. With a small probe size (∼25 μm), the Micro-funnel can be implanted into live multicellular tumor spheroids to accumulate the extracellular metabolites produced by cancer cells. Metabolites collected in the Micro-funnel device were then extracted by a microscale sampling and ionization device, the Single-probe, for real-time mass spectrometry (MS) analysis. We successfully detected the abundance change of anticancer drug irinotecan and its metabolites inside spheroids treated under a series of conditions. Moreover, we found that irinotecan treatment dramatically altered the composition of extracellular compounds. Specifically, we observed the increased abundances of a large number of lipids, which are potentially related to the drug resistance of cancer cells. This study provides a novel way to detect the extracellular compounds inside live spheroids, and the successful development of our technique can benefit the research in multiple areas, including the microenvironment inside live tissues, cell-cell communication, biomarker discovery, and drug development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.