Evidence map›Paper›PMID 28752641›Full record

ArticleInfluenza and other respiratory viruses2017

Influenza virus-like particles harboring H9N2 HA and NA proteins induce a protective immune response in chicken.

Xin Li, Houbin Ju, Jian Liu, Dequan Yang, Xinyong Qi, Xianchao Yang, Yafeng Qiu, Jie Zheng, Feifei Ge, Jinping Zhou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Influenza and other respiratory viruses, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Review
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Xin LiVeterinary disease diagnostic center, Shanghai Animal Disease Control Center, Shanghai, China.ORCID 0000-0003-3420-7213
Houbin JuVeterinary disease diagnostic center, Shanghai Animal Disease Control Center, Shanghai, China.
Jian LiuVeterinary disease diagnostic center, Shanghai Animal Disease Control Center, Shanghai, China.
Dequan YangVeterinary disease diagnostic center, Shanghai Animal Disease Control Center, Shanghai, China.
Xinyong QiVeterinary disease diagnostic center, Shanghai Animal Disease Control Center, Shanghai, China.
Xianchao YangVeterinary disease diagnostic center, Shanghai Animal Disease Control Center, Shanghai, China.
Yafeng QiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Jie ZhengCollege of Biological Sciences and Biotechnology, Yangzhou University, Yangzhou, China.
Feifei GeVeterinary disease diagnostic center, Shanghai Animal Disease Control Center, Shanghai, China.
Jinping ZhouVeterinary disease diagnostic center, Shanghai Animal Disease Control Center, Shanghai, China.
China Animal Disease Control Center · CNShanghai Veterinary Research Institute · CNYangzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAvian influenza viruses represent a growing threat of an influenza pandemic. The co-circulation of multiple H9N2 genotypes over the past decade has been replaced by one predominant genotype-G57 genotype, which displays a changed antigenicity and improved adaptability in chickens. Effective H9N2 subtype avian influenza virus vaccines for poultry are urgently needed.

objectiveIn this study, we constructed H9N2 subtype avian influenza virus-like particle (VLP) and evaluated its protective efficacy in specific pathogen-free (SPF) chickens to lay the foundation for developing an effective vaccine against influenza viruses.

methodsExpression of influenza proteins in VLPs was confirmed by Western blot, hemagglutination inhibition (HI), and neuraminidase inhibition (NI). The morphology was observed by electron microscopy. A group of 15 three-week-old SPF chickens was divided into three subgroups of five chickens immunized with VLP, commercial vaccine, and PBS. Challenge study was performed to evaluate efficacy of VLP vaccine. RESULTS AND

conclusionsThe hemagglutinin (HA) and neuraminidase (NA) proteins were co-expressed in the infected cells, self-assembled, and were released into the culture medium in the form of VLPs of diameter ~80 nm. The VLPs exhibited some functional characteristics of a full influenza virus, including hemagglutination and neuraminidase activity. In SPF chickens, the VLPs elicited serum antibodies specific for H9N2 and induced a higher HI titer (as detected by a homologous antigen) than did a commercial H9N2 vaccine (A/chicken/Shanghai/F/1998). Viral shedding from VLP vaccine subgroup was reduced compared with commercial vaccine subgroup and control subgroup.

Indexed as

AnimalsAntibodies, ViralChickensHemagglutination Inhibition TestsHemagglutinin Glycoproteins, Influenza VirusInfluenza A Virus, H9N2 SubtypeInfluenza in BirdsInfluenza VaccinesNeuraminidasePoultry DiseasesSpecific Pathogen-Free OrganismsVaccinationVaccines, InactivatedVaccines, Virus-Like ParticleVirus SheddingAntibodies, ViralHemagglutinin Glycoproteins, Influenza VirusInfluenza VaccinesNeuraminidaseVaccines, InactivatedVaccines, Virus-Like ParticlechickensH9N2vaccinevirus-like particles

Identifiers

PMID28752641
PMCPMC5705689
OpenAlexW2740563900

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.