ArticleBio-protocol2017
Muscle Histology Characterization Using H&E Staining and Muscle Fiber Type Classification Using Immunofluorescence Staining.
Article in Bio-protocol, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
82 citing papers in PubMed.
- Cocoa flavanols, Nrf2 activation, and oxidative stress in peripheral artery disease: mechanistic findings in muscle based on outcomes from a randomized trial.American journal of physiology. Cell physiology · 2024Trial
- Bilateral Muscle Architecture Differences in a Rabbit Model of Unilateral Hindlimb Transtibial Amputation: A Pilot Study.Muscles (Basel, Switzerland) · 2026Article
- Analysis of Differences in Flavor Precursors Between Fast and Slow Muscles of Turpan Black Sheep Based on Lipidomics and Proteomics.Foods (Basel, Switzerland) · 2026Article
- Transcriptomic Profiling Reveals Early Molecular Adaptations to Resistance Exercise During Disuse Muscle Atrophy with Complementary Automated H&E-Based Muscle Morphometry.Life (Basel, Switzerland) · 2026Article
- Regenerative Index: a method to assess muscle regeneration in patients with Duchenne muscular dystrophy.Skeletal muscle · 2026Article
- Chinese cobra (Naja atra) SVPLACommunications biology · 2026Article
- Recombinant Human KAI1/CD82 Attenuates Glucocorticoid-Induced Muscle Atrophy by Promoting Myogenic Differentiation.International journal of molecular sciences · 2026Article
- ASB5 is a specific marker for muscle satellite cells but dispensable for skeletal muscle development and regeneration.Skeletal muscle · 2026Article
- Regenerative Index reveals declining muscle regeneration in paediatric patients with Duchenne muscular dystrophy.bioRxiv : the preprint server for biology · 2026Article
- Conditional Dmd ablation in muscle and brain causes profound effects on muscle function and neurobehavior.Communications biology · 2025Article
- The functions of MicroRNAs in myogenesis and their effects in beef cattle meat quality: a review.Molecular biology reports · 2025Review
- Study on the Development and Formation Specifics of Longissimus Dorsi Muscles in Ziwuling Black Goats.Animals : an open access journal from MDPI · 2025Article
- Article
- Puerarin Ameliorates Sarcopenia in Aged Mice via Modulation of Inflammation and Oxidative Stress: Insights from Proteomics and Transcriptomics.Inflammation · 2025Article
- MyoAnalyst: an ImageJ plugin for accurate and automatic myofiber segmentation and analysis in skeletal muscle cross sections.Histochemistry and cell biology · 2025Article
- Interaction of Programmed Obesity and Postnatal High Fat Diet but Not (-)-Epicatechin Treatment Modifies Muscle Atrophy Proteins Levels in Male Wistar Rats.Cell biochemistry and biophysics · 2025Article
- Growth hormone-deficient Ames dwarf mice resist sarcopenia and exhibit enhanced endurance running performance at 24 months.GeroScience · 2025Article
- Comparative Transcriptomic Analysis Between High- and Low-Growth-Rate Meat-Type Rabbits Reveals Key Pathways Associated with Muscle Development.Animals : an open access journal from MDPI · 2025Article
- Effects of Bioconverted Guava Leaf (International journal of molecular sciences · 2025Article
- Optimizing Confocal Imaging Protocols for Muscle Fiber Typing in the Mouse Masseter Muscle.Bio-protocol · 2025Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
Abstract
Muscle function is determined by its structure and fiber type composition. Here we describe a protocol to examine muscle histology and myofiber types using hematoxylin and eosin (H&E) and immunofluorescence staining, respectively. H&E stain nucleus in blue and cytoplasm in red, therefore allowing for morphological analyses, such as myofiber diameter, the presence of degenerated and regenerated myofibers, and adipocytes and fibrotic cells. Muscle fibers in adult skeletal muscles of rodents are classified into 4 subtypes based on the expression of myosin heavy chain proteins: Myh7 (type I fiber), Myh2 (type IIA fiber), Myh1 (type IIX fiber), Myh4 (type IIB fiber). A panel of monoclonal antibodies can be used to specifically label these muscle fiber subtypes. These protocols are commonly used in the study of muscle development, growth and regeneration (for example: Wang
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