ArticleScientific reports2017
"High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology".
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
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- Surveying Low-Cost Methods to Measure Lifespan and Healthspan in Caenorhabditis elegans.Journal of visualized experiments : JoVE · 2022Article
- Imaging of Actin Cytoskeletal Integrity During Aging in C. elegans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Adhesion-mediated mechanosignaling forces mitohormesis.Cell metabolism · 2021Article
- Divergent Nodes of Non-autonomous UPRCell reports · 2020Article
- Lysosomal recycling of amino acids affects ER quality control.Science advances · 2020Article
- Measurements of Physiological Stress Responses in C. Elegans.Journal of visualized experiments : JoVE · 2020Article
- High-Throughput Small Molecule Screen Identifies Modulators of Mitochondrial Function in Neurons.iScience · 2020Article
- Assessment of Ethanol-Induced Toxicity on iPSC-Derived Human Neurons Using a Novel High-Throughput Mitochondrial Neuronal Health (MNH) Assay.Frontiers in cell and developmental biology · 2020Article
- UPRScience advances · 2020Article
- Autophagy accounts for approximately one-third of mitochondrial protein turnover and is protein selective.Autophagy · 2019Article
- Spatial regulation of the actin cytoskeleton by HSF-1 during aging.Molecular biology of the cell · 2018Article
- Sizing lipid droplets from adult and geriatric mouse liver tissue via nanoparticle tracking analysis.Analytical and bioanalytical chemistry · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The tissue-specific etiology of aging and stress has been elusive due to limitations in data processing of current techniques. Despite that many techniques are high-throughput, they usually use singular features of the data (e.g. whole fluorescence). One technology at the nexus of fluorescence-based screens is large particle flow cytometry ("biosorter"), capable of recording positional fluorescence and object granularity information from many individual live animals. Current processing of biosorter data, however, do not integrate positional information into their analysis and data visualization. Here, we present a bioanalytical platform for the quantification of positional information ("longitudinal profiling") of C. elegans, which we posit embodies the benefits of both high-throughput screening and high-resolution microscopy. We show the use of these techniques in (1) characterizing distinct responses of a transcriptional reporter to various stresses in defined anatomical regions, (2) identifying regions of high mitochondrial membrane potential in live animals, (3) monitoring regional mitochondrial activity in aging models and during development, and (4) screening for regulators of muscle mitochondrial dynamics in a high-throughput format. This platform offers a significant improvement in the quality of high-throughput biosorter data analysis and visualization, opening new options for region-specific phenotypic screening of complex physiological phenomena and mitochondrial biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.