ArticlePloS one2017
Gene expression and metabolite profiling of gibberellin biosynthesis during induction of somatic embryogenesis in Medicago truncatula Gaertn.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- The multifaceted role of gibberellins in somatic embryogenesis: fromFrontiers in plant science · 2026Review
- Gibberellins involved in fruit ripening and softening by mediating multiple hormonal signals in tomato.Horticulture research · 2024Article
- Genomic and Transcriptomic Analysis of Pea (Plants (Basel, Switzerland) · 2023Article
- Transcriptomic Profiling of Embryogenic and Non-Embryogenic Callus Provides New Insight into the Nature of Recalcitrance in Cannabis.International journal of molecular sciences · 2023Article
- Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus ofCurrent issues in molecular biology · 2023Article
- Regulatory networks of hormone-involved transcription factors and their downstream pathways during somatic embryogenesis of3 Biotech · 2023Article
- A genetic screen for dominant chloroplast reactive oxygen species signaling mutants reveals life stage-specific singlet oxygen signaling networks.Frontiers in plant science · 2023Article
- Manipulating GA-Related Genes for Cereal Crop Improvement.International journal of molecular sciences · 2022Review
- Article
- Current Proteomic and Metabolomic Knowledge of Zygotic and Somatic Embryogenesis in Plants.International journal of molecular sciences · 2021Review
- Salt Stress Enhances Early Symbiotic Gene Expression inMolecular plant-microbe interactions : MPMI · 2021Article
- Article
- The GA 20-Oxidase Encoding GeneFrontiers in plant science · 2021Article
- Cloning and Functional Analysis of Dwarf GeneInternational journal of molecular sciences · 2020Article
- Effects of genotypes and explants on garlic callus production and endogenous hormones.Scientific reports · 2020Article
- MtGA2ox10 encoding C20-GA2-oxidase regulates rhizobial infection and nodule development in Medicago truncatula.Scientific reports · 2019Article
- Somatic Embryogenesis in theFrontiers in plant science · 2019Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gibberellins (GAs) are involved in the regulation of numerous developmental processes in plants including zygotic embryogenesis, but their biosynthesis and role during somatic embryogenesis (SE) is mostly unknown. In this study we show that during three week- long induction phase, when cells of leaf explants from non-embryogenic genotype (M9) and embryogenic variant (M9-10a) were forming the callus, all the bioactive gibberellins from non-13-hydroxylation (GA4, GA7) and 13-hydroxylation (GA1, GA5, GA3, GA6) pathways were present, but the contents of only a few of them differed between the tested lines. The GA53 and GA19 substrates synthesized by the 13-hydroxylation pathway accumulated specifically in the M9-10a line after the first week of induction; subsequently, among the bioactive gibberellins detected, only the content of GA3 increased and appeared to be connected with acquisition of embryogenic competence. We fully annotated 20 Medicago truncatula orthologous genes coding the enzymes which catalyze all the known reactions of gibberellin biosynthesis. Our results indicate that, within all the genes tested, expression of only three: MtCPS, MtGA3ox1 and MtGA3ox2, was specific to embryogenic explants and reflected the changes observed in GA53, GA19 and GA3 contents. Moreover, by analyzing expression of MtBBM, SE marker gene, we confirmed the inhibitory effect of manipulation in GAs metabolism, applying exogenous GA3, which not only impaired the production of somatic embryos, but also significantly decreased expression of this gene.
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