ArticlePloS one2017
Nanofluidic digital PCR for the quantification of Norovirus for water quality assessment.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Drivers of the paradigm shift in norovirus diagnostics: technological innovation, contextual demands, and collaborative synergy.Virology journal · 2026Review
- Review
- Article
- Environmental monitoring of SARS-CoV-2 in the metropolitan area of Porto Alegre, Rio Grande do Sul (RS), Brazil.Environmental science and pollution research international · 2024Article
- Molecular and Genetics-Based Systems for Tracing the Evolution and Exploring the Mechanisms of Human Norovirus Infections.International journal of molecular sciences · 2023Review
- Wastewater-based epidemiological investigation of SARS-CoV-2 in Porto Alegre, Southern Brazil.Science in One Health · 2022Article
- Recovery of SARS-CoV-2 from large volumes of raw wastewater is enhanced with the inuvai R180 system.Journal of environmental management · 2022Article
- Advances and Future Perspective on Detection Technology of Human Norovirus.Pathogens (Basel, Switzerland) · 2021Review
- Application of One-Step Reverse Transcription Droplet Digital PCR for Dengue Virus Detection and Quantification in Clinical Specimens.Diagnostics (Basel, Switzerland) · 2021Article
- COVID-19 Crisis Creates Opportunity towards Global Monitoring & Surveillance.Pathogens (Basel, Switzerland) · 2021Review
- Microbial Indicators of Fecal Pollution: Recent Progress and Challenges in Assessing Water Quality.Current environmental health reports · 2020Review
- SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area.Water research · 2020Article
- Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus.Frontiers in veterinary science · 2020Article
- Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field.Frontiers in microbiology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sensitive detection of water- and foodborne enteric viruses is extremely relevant, especially due to the low concentrations in which they are found. Accurate and sensitive detection of Norovirus, the primary responsible for water- and foodborne outbreaks, is of particular importance. Quantification of Norovirus is commonly performed by quantitative RT-PCR (RT-qPCR). In recent years a new platform was developed, digital PCR, that quantifies without the need for a standard curve thus decreasing the errors associated with its utilization. The platform developed by LifeTechnologies, QuantStudio 3D Digital PCR is amongst the least studied digital platform and although it allows the direct detection of DNA targets it requires a two-step RT-PCR for the detection of RNA targets. In this work we developed a new protocol able to detect Norovirus using a one-step digital PCR reaction (RT-dPCR). The performance of the newly developed one-step digital PCR was compared to RT-qPCR for the detection of Norovirus genogroup I and genogroup II. The sensitivity of RT-dPCR was identical to that of RT-qPCR, and the quantitative data determined by both methods were not significantly different for most samples. This one-step absolute quantification approach is a useful tool to minimize the time spent currently using this particular platform to amplify viral RNA and to standardize quantification of enteric viruses in food and environmental samples. This study proved the usefulness of the newly developed RT-dPCR protocol for a sensitive and accurate detection of low-copy targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.