ArticlePloS one2017
Investigating asymmetric salt profiles for nanopore DNA sequencing with biological porin MspA.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Nanopore-enabled time-resolved monitoring of catecholamine-related phenylalanine metabolism.Nature nanotechnology · 2026Article
- Structural and functional insights into acidophilic helicases as DNA-unwinding motors.Applied and environmental microbiology · 2025Article
- Vectorial Discrimination of Small Molecules with a Macrocycle Adaptor-Protein Nanopore System and Nanocavity-Dependent, pH Gradient-Controlled Analyte Kinetics.Analytical chemistry · 2025Article
- Nanopores map the acid-base properties of a single site in a single DNA molecule.Nucleic acids research · 2024Article
- High-throughput single biomarker identification using droplet nanopore.Chemical science · 2024Article
- Controllable Fabrication of Sub-10 nm Graphene Nanopores via Helium Ion Microscopy and DNA Detection.Biosensors · 2024Article
- Control of subunit stoichiometry in single-chain MspA nanopores.Biophysical journal · 2022Article
- Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants.RSC advances · 2021Review
- Systemic Overview of Microstrip Patch Antenna's for Different Biomedical Applications.Advanced pharmaceutical bulletin · 2021Review
- Modelling single-molecule kinetics of helicase translocation using high-resolution nanopore tweezers (SPRNT).Essays in biochemistry · 2021Review
- Biological Nanopores: Engineering on Demand.Life (Basel, Switzerland) · 2021Review
- Inverted duplicate DNA sequences increase translocation rates through sequencing nanopores resulting in reduced base calling accuracy.Nucleic acids research · 2020Article
- Conformational fluctuations of a DNA electrophoretically translocating through a nanopore under the action of a motor protein.The European physical journal. E, Soft matter · 2019Article
- Fabrication and Applications of Solid-State Nanopores.Sensors (Basel, Switzerland) · 2019Review
- Slowing down DNA translocation velocity using a LiCl salt gradient and nanofiber mesh.European biophysics journal : EBJ · 2019Article
- Direct sequencing of 2'-deoxy-2'-fluoroarabinonucleic acid (FANA) using nanopore-induced phase-shift sequencing (NIPSS).Chemical science · 2019Article
- FraC nanopores with adjustable diameter identify the mass of opposite-charge peptides with 44 dalton resolution.Nature communications · 2019Article
- Nanopore label-free detection of single-nucleotide deletion in Baxα/BaxΔ2.Electrophoresis · 2018Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Nanopore DNA sequencing is a promising single-molecule analysis technology. This technique relies on a DNA motor enzyme to control movement of DNA precisely through a nanopore. Specific experimental buffer conditions are required based on the preferred operating conditions of the DNA motor enzyme. While many DNA motor enzymes typically operate in salt concentrations under 100 mM, salt concentration simultaneously affects signal and noise magnitude as well as DNA capture rate in nanopore sequencing, limiting standard experimental conditions to salt concentrations greater than ~100 mM in order to maintain adequate resolution and experimental throughput. We evaluated the signal contribution from ions on both sides of the membrane (cis and trans) by varying cis and trans [KCl] independently during phi29 DNA Polymerase-controlled translocation of DNA through the biological porin MspA. Our studies reveal that during DNA translocation, the negatively charged DNA increases cation selectivity through MspA with the majority of current produced by the flow of K+ ions from trans to cis. Varying trans [K+] has dramatic effects on the signal magnitude, whereas changing cis [Cl-] produces only small effects. Good signal-to-noise can be maintained with cis [Cl-] as small as 20 mM, if the concentration of KCl on the trans side is kept high. These results demonstrate the potential of using salt-sensitive motor enzymes (helicases, polymerases, recombinases) in nanopore systems and offer a guide for selecting buffer conditions in future experiments to simultaneously optimize signal, throughput, and enzyme activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.