ArticlePloS one2017
Simultaneous detection of three pome fruit tree viruses by one-step multiplex quantitative RT-PCR.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Transmission of apple stem grooving virus (Capillovirus mali) to apple from the soil-borne fungus Fusarium solani.BMC plant biology · 2025Article
- Comprehensive Virome Profiling of Apple Mosaic Disease-Affected Trees in Iran Using RT-PCR and Next-Generation Sequencing.Viruses · 2025Article
- Development of a TaqMan qPCR for the Simultaneous Detection of the TuMV and BBWV2 Viruses Responsible for the Viral Disease inMicroorganisms · 2024Article
- Article
- A novel study on bean common mosaic virus accumulation shows disease resistance at the initial stage of infection inFrontiers in genetics · 2023Article
- Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR.The plant pathology journal · 2022Article
- Metatranscriptomic Analysis of Plant Viruses in Imported Pear and Kiwifruit Pollen.The plant pathology journal · 2022Article
- Viruses Infecting Trees and Herbs That Produce Edible Fleshy Fruits with a Prominent Value in the Global Market: An Evolutionary Perspective.Plants (Basel, Switzerland) · 2022Review
- First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain.Plants (Basel, Switzerland) · 2021Article
- Field detection of multiple RNA viruses/viroids in apple using a CRISPR/Cas12a-based visual assay.Plant biotechnology journal · 2021Article
- Loquat (Plants (Basel, Switzerland) · 2020Article
- Detection of Plant Viruses and Disease Management: Relevance of Genetic Diversity and Evolution.Frontiers in plant science · 2020Review
- Establishment of a Sensitive qPCR Methodology for Detection of the Olive-Infecting Viruses in Portuguese and Tunisian Orchards.Frontiers in plant science · 2019Article
- Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies.Viruses · 2018Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A one-step multiplex real-time reverse transcription polymerase chain reaction (RT-qPCR) based on TaqMan probes was developed for the simultaneous detection of Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in total RNA of pome trees extracted with a CTAB method. The sensitivity of the method was established using in vitro synthesized viral transcripts serially diluted in RNA from healthy, virus-tested (negative) pome trees. The three viruses were simultaneously detected up to a 10-4 dilution of total RNA from a naturally triple-infected apple tree prepared in total RNA of healthy apple tissue. The newly developed RT-qPCR assay was at least one hundred times more sensitive than conventional single RT-PCRs. The assay was validated with 36 field samples for which nine triple and 11 double infections were detected. All viruses were detected simultaneously in composite samples at least up to the ratio of 1:150 triple-infected to healthy pear tissue, suggesting the assay has the capacity to examine rapidly a large number of samples in pome tree certification programs and surveys for virus presence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.