ArticlePLoS genetics2017
Krox20 hindbrain regulation incorporates multiple modes of cooperation between cis-acting elements.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Rapgef1 paralog-mediated regulation of Wnt/β-catenin signaling orchestrates early embryo tissue patterning and morphogenesis.Cell communication and signaling : CCS · 2026Article
- Temporal constraints on enhancer usage shape the regulation of limb gene transcription.Nature communications · 2026Article
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- Sea lamprey enlightens the origin of the coupling of retinoic acid signaling to vertebrate hindbrain segmentation.Nature communications · 2024Article
- A CRISPR/Cas9-engineered mouse carrying a conditional knockout allele for the early growth response-1 transcription factor.Genesis (New York, N.Y. : 2000) · 2023Article
- Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids.Communications biology · 2023Article
- The HDAC7-TET2 epigenetic axis is essential during early B lymphocyte development.Nucleic acids research · 2022Article
- A fast Myosin super enhancer dictates muscle fiber phenotype through competitive interactions with Myosin genes.Nature communications · 2022Article
- Multiple morphogens and rapid elongation promote segmental patterning during development.PLoS computational biology · 2021Article
- CTCF modulates allele-specific sub-TAD organization and imprinted gene activity at the mouse Dlk1-Dio3 and Igf2-H19 domains.Genome biology · 2019Article
- Hindbrain induction and patterning during early vertebrate development.Cellular and molecular life sciences : CMLS · 2019Review
- Cooperation, cis-interactions, versatility and evolutionary plasticity of multiple cis-acting elements underlie krox20 hindbrain regulation.PLoS genetics · 2018Article
- Targeted Enhancer Activation by a Subunit of the Integrator Complex.Molecular cell · 2018Article
- Establishing sharp and homogeneous segments in the hindbrain.F1000Research · 2018Review
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developmental genes can harbour multiple transcriptional enhancers that act simultaneously or in succession to achieve robust and precise spatiotemporal expression. However, the mechanisms underlying cooperation between cis-acting elements are poorly documented, notably in vertebrates. The mouse gene Krox20 encodes a transcription factor required for the specification of two segments (rhombomeres) of the developing hindbrain. In rhombomere 3, Krox20 is subject to direct positive feedback governed by an autoregulatory enhancer, element A. In contrast, a second enhancer, element C, distant by 70 kb, is active from the initiation of transcription independent of the presence of the KROX20 protein. Here, using both enhancer knock-outs and investigations of chromatin organisation, we show that element C possesses a dual activity: besides its classical enhancer function, it is also permanently required in cis to potentiate the autoregulatory activity of element A, by increasing its chromatin accessibility. This work uncovers a novel, asymmetrical, long-range mode of cooperation between cis-acting elements that might be essential to avoid promiscuous activation of positive autoregulatory elements.
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