ArticleeLife2017
Super-resolution imaging of synaptic and Extra-synaptic AMPA receptors with different-sized fluorescent probes.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 77 citations in OpenAlex.
- Calcium dynamics in small spaces: Lessons learned from modeling in dendritic spines.Biophysical journal · 2025Review
- Distinct transmission sites within a synapse for strengthening and homeostasis.Science advances · 2025Article
- Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors.Nature communications · 2024Article
- Nanoscale organization is changed in native, surface AMPARs by mouse brain region and tauopathy.bioRxiv : the preprint server for biology · 2024Article
- Synapse-type-specific competitive Hebbian learning forms functional recurrent networks.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Versatile Prepolymer Platform for Controlled Tailoring of Quantum Dot Surface Properties.ACS applied materials & interfaces · 2024Article
- Quantum Dot Fluorescent Imaging: Using Atomic Structure Correlation Studies to Improve Photophysical Properties.The journal of physical chemistry. C, Nanomaterials and interfaces · 2024Review
- A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments.Cell reports methods · 2023Article
- Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Afferent convergence to a shared population of interneuron AMPA receptors.Nature communications · 2023Article
- Quantitative DNA-PAINT imaging of AMPA receptors in live neurons.Cell reports methods · 2023Article
- AMPA and GABAA receptor nanodomains assemble in the absence of synaptic neurotransmitter release.Frontiers in molecular neuroscience · 2023Article
- Structural Design of Multidentate Copolymers as Compact Quantum Dot Coatings for Live-Cell Single-Particle Imaging.Chemistry of materials : a publication of the American Chemical Society · 2022Article
- Monitoring Various Bioactivities at the Molecular, Cellular, Tissue, and Organism Levels via Biological Lasers.Sensors (Basel, Switzerland) · 2022Review
- The Shaping of AMPA Receptor Surface Distribution by Neuronal Activity.Frontiers in synaptic neuroscience · 2022Review
- Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors.eLife · 2021Article
- Lack of support for surface diffusion of postsynaptic AMPARs in tuning synaptic transmission.Biophysical journal · 2021Article
- Antibody Self-Assembly Maximizes Cytoplasmic Immunostaining Accuracy of Compact Quantum Dots.Chemistry of materials : a publication of the American Chemical Society · 2021Article
- Advanced imaging and labelling methods to decipher brain cell organization and function.Nature reviews. Neuroscience · 2021Review
- Reciprocal stabilization of glycine receptors and gephyrin scaffold proteins at inhibitory synapses.Biophysical journal · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 3 institutions in 1 country.
Funding
Abstract
Previous studies tracking AMPA receptor (AMPAR) diffusion at synapses observed a large mobile extrasynaptic AMPAR pool. Using super-resolution microscopy, we examined how fluorophore size and photostability affected AMPAR trafficking outside of, and within, post-synaptic densities (PSDs) from rats. Organic fluorescent dyes (≈4 nm), quantum dots, either small (≈10 nm diameter; sQDs) or big (>20 nm; bQDs), were coupled to AMPARs via different-sized linkers. We find that >90% of AMPARs labeled with fluorescent dyes or sQDs were diffusing in confined nanodomains in PSDs, which were stable for 15 min or longer. Less than 10% of sQD-AMPARs were extrasynaptic and highly mobile. In contrast, 5-10% of bQD-AMPARs were in PSDs and 90-95% were extrasynaptic as previously observed. Contrary to the hypothesis that AMPAR entry is limited by the occupancy of open PSD 'slots', our findings suggest that AMPARs rapidly enter stable 'nanodomains' in PSDs with lifetime
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.