ArticleeLife2017
Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers.
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Who cites it
124 citing papers in PubMed.
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- Protein Surface Site Determines the Evolutionary Accessibility of Allosteric Regulation.bioRxiv : the preprint server for biology · 2026Article
- Undoing of firing rate adaptation enables invariant population codes.Science advances · 2026Article
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- FACED 2.0 enables large-scale voltage and calcium imaging in vivo.Nature methods · 2026Article
- A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.bioRxiv : the preprint server for biology · 2026Article
- Improving positively tuned voltage indicators for faster kinetics and higher contrast.bioRxiv : the preprint server for biology · 2026Article
- Designer indicators for two-photon recording of subthreshold voltage dynamics.Nature methods · 2026Article
- Voltage imaging as a window into neural computation.Neurophotonics · 2026Article
- Multimodal optical imaging combining voltage-sensitive dye ElectroFluor630 with genetically encoded calcium, glutamate, or voltage indicators.Neurophotonics · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- An essential experimental control for functional connectivity mapping with optogenetics.Genetics · 2025Article
- Multi-photon, label-free photoacoustic and optical imaging of NADH in brain cells.Light, science & applications · 2025Article
- Twists to Classical Conditioning of AdultCold Spring Harbor protocols · 2025Article
- Fluorescent sensors for intracellular signaling in the brain: imaging neurons, glia, and vascular cells.Neurophotonics · 2025Article
- Genetically encoded sensors illuminateIBRO neuroscience reports · 2025Article
- Deep-Penetrating and High-Resolution Continuous-Wave Nonlinear Microscopy Based on Homologous Dual-Emission Upconversion Adaptive Optics.Nano letters · 2025Article
- Principles and Design of Molecular Tools for Sensing and Perturbing Cell Surface Receptor Activity.Chemical reviews · 2025Review
- Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators.Science advances · 2025Article
- Ultrafast optical imaging techniques for exploring rapid neuronal dynamics.Neurophotonics · 2025Review
64 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
Monitoring voltage dynamics in defined neurons deep in the brain is critical for unraveling the function of neuronal circuits but is challenging due to the limited performance of existing tools. In particular, while genetically encoded voltage indicators have shown promise for optical detection of voltage transients, many indicators exhibit low sensitivity when imaged under two-photon illumination. Previous studies thus fell short of visualizing voltage dynamics in individual neurons in single trials. Here, we report ASAP2s, a novel voltage indicator with improved sensitivity. By imaging ASAP2s using random-access multi-photon microscopy, we demonstrate robust single-trial detection of action potentials in organotypic slice cultures. We also show that ASAP2s enables two-photon imaging of graded potentials in organotypic slice cultures and in
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