ArticleActa pharmacologica Sinica2018
A novel and quick PCR-based method to genotype mice with a leptin receptor mutation (db/db mice).
Article in Acta pharmacologica Sinica, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
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- An integrated approach to predict genetic risk for Mosquito-Borne diseases in the local Population of Tehsil Haripur, Khyber Pakhtunkhwa, Pakistan.Scientific reports · 2025Article
- The glucocorticoid receptor as a master regulator of the Müller cell response to diabetic conditions in mice.Journal of neuroinflammation · 2024Article
- Animal models for type 1 and type 2 diabetes: advantages and limitations.Frontiers in endocrinology · 2024Review
- Rejection of inappropriate synaptic partners in mouse retina mediated by transcellular FLRT2-UNC5 signaling.Developmental cell · 2023Article
- DetectingHeliyon · 2023Article
- Characterization of the ZebrafishCells · 2023Article
- Tetra-Primer Amplification-Refractory Mutation System (ARMS)-PCR for Genotyping Mouse Leptin Gene Mutation.Animals : an open access journal from MDPI · 2022Article
- Investigating the Expression and Function of the Glucose Transporter GLUT6 in Obesity.International journal of molecular sciences · 2022Article
- Association of Variants inThe Malaysian journal of medical sciences : MJMS · 2022Article
- The PPARγ agonist pioglitazone produces a female-predominant inhibition of hyperalgesia associated with surgical incision, peripheral nerve injury, and painful diabetic neuropathy.Neuropharmacology · 2022Article
- Article
- Protective effect of carvacrol on liver injury in type 2 diabetic db/db mice.Molecular medicine reports · 2021Article
- Development of heart failure with preserved ejection fraction in type 2 diabetic mice is ameliorated by preserving vascular function.Life sciences · 2021Article
- Carvacrol may alleviate vascular inflammation in diabetic db/db mice.International journal of molecular medicine · 2020Article
- MG53 Does Not Manifest the Development of Diabetes inDiabetes · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
db/db mice is one of most widely used animal models in studying the cellular and molecular mechanisms of metabolic disorders, such as diabetes, hyperlipidemia, and obesity. The mice carry spontaneous point mutations in the gene encoding the leptin receptor, leading to leptin receptor inactivation. Since homozygous db/db mice are sterile, the maintenance of db/db mice requires breeding between heterozygous pairs, which makes genotyping essential for the identification of offspring. The aim of this study was to develop a quick and highly repeatable method for genotyping db/db mice, which comprised only three simple steps: genomic DNA is extracted from either tail tips or ear notches via alkaline lysis (∼20 min); samples are then subjected to tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) using specially designed and validated primer sets (∼1.5 h); finally, genotypes are be determined by resolving PCR products on regular DNA electrophoresis (∼10 min). The entire db/db mice genotyping procedure can be performed using regular Taq polymerase and PCR amplification within 2 h. Other advantages of this method include high sensitivity and reproducibility. Minimal amounts of tissue from mice are required, and genomic DNA samples can be stably stored at room temperature for up to one month. In conclusion, the method is simple, cost effective, sensitive and reliable, which will greatly facilitate studies using db/db mice.
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