ArticleScientific reports2017
Deformability based Cell Sorting using Microfluidic Ratchets Enabling Phenotypic Separation of Leukocytes Directly from Whole Blood.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Ultra-low extracorporeal volume microfluidic leukapheresis is safe and effective in a rat model.Nature communications · 2025Article
- Robust and efficient separation of white blood cells from blood using a microfluidic chip with a pair of linearly tapered crossflow filter arrays.Mikrochimica acta · 2024Article
- Biophysical profiling of red blood cells from thin-film blood smears using deep learning.Heliyon · 2024Article
- Biomechanics of circulating cellular and subcellular bioparticles: beyond separation.Cell communication and signaling : CCS · 2024Review
- Rapid, label-free enrichment of lymphocytes in a closed system using a flow-through microfluidic device.Bioengineering & translational medicine · 2024Article
- Recent advances in microfluidic cell separation to enable centrifugation-free, low extracorporeal volume leukapheresis in pediatric patients.Blood transfusion = Trasfusione del sangue · 2023Review
- Data for assessing red blood cell deformability from microscopy images using deep learning.Data in brief · 2023Article
- Article
- Collective transient ratchet transport induced by many elastically interacting particles.Scientific reports · 2021Article
- Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics.Lab on a chip · 2020Article
- Changes of Inertial Focusing Position in a Triangular Channel Depending on Droplet Deformability and Size.Micromachines · 2020Article
- Effect of elastic modulus on inertial displacement of cell-like particles in microchannels.Biomicrofluidics · 2020Article
- Microfluidic assessment of red blood cell mediated microvascular occlusion.Lab on a chip · 2020Article
- Etching of Uncompensated Convex Corners with Sides along <n10> and <100> in 25 wt% TMAH at 80 °C.Micromachines · 2020Article
- Cell trapping in Y-junction microchannels: A numerical study of the bifurcation angle effect in inertial microfluidics.Physics of fluids (Woodbury, N.Y. : 1994) · 2019Article
- Microfluidic cell sorting: Towards improved biocompatibility of extracorporeal lung assist devices.Scientific reports · 2018Article
- Rapid and effective enrichment of mononuclear cells from blood using acoustophoresis.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The separation of leukocytes from whole blood is a prerequisite for many biological assays. Traditional methods require significant sample volumes and are often undesirable because they expose leukocytes to harsh physical or chemical treatment. Existing microfluidic approaches can work with smaller volumes, but lack selectivity. In particular, the selectivity of microfluidic systems based on microfiltration is limited by fouling due to clogging. Here, we developed a method to separate leukocytes from whole blood using the microfluidic ratchet mechanism, which filters the blood sample using a matrix of micrometer-scale tapered constrictions. Deforming single cells through such constrictions requires directionally asymmetrical forces, which enables oscillatory flow to create a ratcheting transport that depends on cell size and deformability. Simultaneously, oscillatory flow continuously agitates the cells to limit the contact time with the filter microstructure to prevent adsorption and clogging. We show this device is capable of isolating leukocytes from whole blood with 100% purity (i.e. no contaminant erythrocytes) and <2% leukocytes loss. We further demonstrate the potential to phenotypically sort leukocytes to enrich for granulocytes and lymphocytes subpopulations. Together, this process provides a sensitive method to isolate and sort leukocytes directly from whole blood based on their biophysical properties.
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