ArticleRNA (New York, N.Y.)2017
Ribonucleoprotein purification and characterization using RNA Mango.
Article in RNA (New York, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 30 citations in OpenAlex.
- Live imaging and interactome analysis of Zika and chikungunya viral RNAs via dual-action aptamer tag.Nature communications · 2026Article
- A multivalent RNA affinity tag enables selective purification of tagged RNAs and bound proteins.Nucleic acids research · 2026Article
- RNA aptamers as tools for the purification and analysis of in vivo assembled ribonucleoproteins.RNA (New York, N.Y.) · 2025Review
- Live Imaging and Interactome Analysis of Zika and Chikungunya Viral RNAs Via Dual-Action Aptamer Tag.Research square · 2025Article
- Transcription factors induce differential splicing of duplicated ribosomal protein genes during meiosis.Nucleic acids research · 2025Article
- Symmetry breaking of fluorophore binding to a G-quadruplex generates an RNA aptamer with picomolar KD.Nucleic acids research · 2024Article
- Methods that shaped telomerase research.Biogerontology · 2024Review
- Turn-on RNA Mango Beacons forRNA (New York, N.Y.) · 2024Article
- Purification of In Vivo or In Vitro-Assembled RNA-Protein Complexes by RNA Centric Methods.Advances in experimental medicine and biology · 2024Article
- Probing the conformational changes ofFrontiers in molecular biosciences · 2023Article
- Visualizing the Nucleome Using the CRISPR-Cas9 System: From in vitro to in vivo.Biochemistry. Biokhimiia · 2023Review
- Article
- Unveiling mRNP composition by fluorescence correlation and cross-correlation spectroscopy using cell lysates.Nucleic acids research · 2021Article
- Following the messenger: Recent innovations in live cell single molecule fluorescence imaging.Wiley interdisciplinary reviews. RNA · 2020Review
- Nanomechanics and co-transcriptional folding of Spinach and Mango.Nature communications · 2019Article
- Ribonucleoprotein Capture by in Vivo Expression of a Designer Pentatricopeptide Repeat Protein in Arabidopsis.The Plant cell · 2019Article
- Highly efficient in vitro translation of authentic affinity-purified messenger ribonucleoprotein complexes.RNA (New York, N.Y.) · 2018Article
- The Lsm1-7/Pat1 complex binds to stress-activated mRNAs and modulates the response to hyperosmotic shock.PLoS genetics · 2018Article
- Purification of RNA Mango Tagged Native RNA-protein Complexes from Cellular Extracts Using TO1-Desthiobiotin Fluorophore Ligand.Bio-protocol · 2018Article
- RNA2DMut: a web tool for the design and analysis of RNA structure mutations.RNA (New York, N.Y.) · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The characterization of RNA-protein complexes (RNPs) is a difficult but increasingly important problem in modern biology. By combining the compact RNA Mango aptamer with a fluorogenic thiazole orange desthiobiotin (TO1-Dtb or TO3-Dtb) ligand, we have created an RNA tagging system that simplifies the purification and subsequent characterization of endogenous RNPs. Mango-tagged RNP complexes can be immobilized on a streptavidin solid support and recovered in their native state by the addition of free biotin. Furthermore, Mango-based RNP purification can be adapted to different scales of RNP isolation ranging from pull-down assays to the isolation of large amounts of biochemically defined cellular RNPs. We have incorporated the Mango aptamer into the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.