Evidence map›Paper›PMID 28746874›Full record

ArticleCell reports2017

Glycolytic Enzymes Coalesce in G Bodies under Hypoxic Stress.

Meiyan Jin, Gregory G Fuller, Ting Han, Yao Yao, Amelia F Alessi, Mallory A Freeberg, Nathan P Roach, James J Moresco, Alla Karnovsky, Misuzu Baba and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.

0numbers the graph read from it
0cells of the map it votes in
103citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

103 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026
    Review
  7. Review
  8. Review
  9. Direct energy consumption by inhibitory GABAFrontiers in neuroscience · 2026
    Article
  10. Article
  11. Article
  12. Review
  13. The metabolic enzyme GYS1 condenses with NONO/p54Cell death and differentiation · 2025
    Article
  14. Review
  15. Review
  16. Article
  17. Review
  18. Article
  19. Review
  20. Review

43 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Meiyan JinLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Gregory G FullerDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Ting HanLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Yao YaoLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.
Amelia F AlessiLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Mallory A FreebergLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Nathan P RoachDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
James J MorescoDepartment of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Alla KarnovskyDepartment of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA.
Misuzu BabaResearch Institute for Science and Technology, Kogakuin University, Hachioji, Tokyo 192-0015, Japan.
John R YatesDepartment of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Aaron D GitlerDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Ken InokiLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Division of Nephrology, Department of Molecular and Integrative Physiology and the Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.
Daniel J KlionskyLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: klionsky@umich.edu.
John K KimLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA. Electronic address: jnkim@jhu.edu.

Funding

ZDS2P REGULATES SWE1P-DEPENDENT POLARIZED CELL GROWTH IN S CEREVISIAEP41RR011823 · NCRR · UNIVERSITY OF WASHINGTON · PI FIELDS, STANLEY · 1996 to 2011
$24.7M
TrainingP41GM103533 · NIGMS · UNIVERSITY OF WASHINGTON · PI YATES III, JOHN R · 2012 to 2021
$20.7M
CELLULAR AND MOLECULAR BIOLOGYT32GM007231 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI VAN DOREN, MARK B · 1985 to 2020
$18.4M
ALTERNATIVE VACUOLAR TARGETING MECHANISMS IN YEASTR01GM053396 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KLIONSKY, DANIEL J. · 1995 to 2018
$12.1M
PREDOCTORAL TRAINING IN GENETICST32GM007544 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MORAN, JOHN V. · 1985 to 2022
$11.3M
Statistics and Bioinformatics Core - WangU24DK097153 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BURANT, CHARLES F · 2012 to 2017
$10.1M
Role of TSC-mTORC1 pathway for podocyte injury in diabetic nephropathyR01DK083491 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI INOKI, KEN · 2009 to 2017
$3.0M
Lysosomal cholesterol-dependent anabolic regulationR01DK124709 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI INOKI, KEN · 2022 to 2025
$2.1M
The role of post-translational modifications in miRISC functionR01GM118875 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI KIM, JOHN · 2016 to 2019
$1.4M
Regulation of Gene Expression by microRNAs in C. elegansR01GM088565 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KIM, JOHN · 2009 to 2013
$1.4M
Molecular mechanism of mTORC1-dependent translation and ribosome biogenesisR01GM110019 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI INOKI, KEN · 2015 to 2018
$1.2M
NCRR NIH HHS P41 RR011823NIDDK NIH HHS R01 DK083491NIDDK NIH HHS R01 DK124709NIDDK NIH HHS U24 DK097153NIGMS NIH HHS P41 GM103533NIGMS NIH HHS R01 GM053396NIGMS NIH HHS R01 GM088565NIGMS NIH HHS R01 GM110019NIGMS NIH HHS R01 GM118875NIGMS NIH HHS T32 GM007231NIGMS NIH HHS T32 GM007544
6 · The paper itself

Abstract

Glycolysis is upregulated under conditions such as hypoxia and high energy demand to promote cell proliferation, although the mechanism remains poorly understood. We find that hypoxia in Saccharomyces cerevisiae induces concentration of glycolytic enzymes, including the Pfk2p subunit of the rate-limiting phosphofructokinase, into a single, non-membrane-bound granule termed the "glycolytic body" or "G body." A yeast kinome screen identifies the yeast ortholog of AMP-activated protein kinase, Snf1p, as necessary for G-body formation. Many G-body components identified by proteomics are required for G-body integrity. Cells incapable of forming G bodies in hypoxia display abnormal cell division and produce inviable daughter cells. Conversely, cells with G bodies show increased glucose consumption and decreased levels of glycolytic intermediates. Importantly, G bodies form in human hepatocarcinoma cells in hypoxia. Together, our results suggest that G body formation is a conserved, adaptive response to increase glycolytic output during hypoxia or tumorigenesis.

Indexed as

Chromatography, LiquidGlucoseGlycolysisHep G2 CellsHumansHypoxiaImmunoprecipitationMass SpectrometryMicroscopy, Electron, TransmissionMicroscopy, FluorescenceRNA-Binding ProteinsGlucoseRNA-Binding Proteinsglycolysishypoxiaintrinsically disordered regionphase transitionsRNA binding proteinRNA granule

Identifiers

PMID28746874
PMCPMC5586494

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.