ArticlePloS one2017
Selection and validation of reference genes for quantitative real-time PCR analysis under different experimental conditions in the leafminer Liriomyza trifolii (Diptera: Agromyzidae).
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
- Bioactivity and Molecular Responses of Bitter Gourd Leaf Extracts Against the Invasive Leafminer,Insects · 2026Article
- Screening optimum reference genes for quantitative real-time polymerase chain reaction analysis in invasive apple snail, Pomacea canaliculata under different conditions.Molecular biology reports · 2026Article
- Reference gene identification and evaluation for accurate qPCR normalization in Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae).Molecular biology reports · 2025Article
- Integrated transcriptional and biochemical profiling suggests mechanisms associated with rapid cold hardening in adult Liriomyza trifolii (Burgess).Scientific reports · 2024Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR Analysis in Cockroach ParasitoidInsects · 2024Article
- Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted BollwormInternational journal of molecular sciences · 2023Article
- Selection and validation of optimal reference genes for RT-qPCR analyses inFrontiers in physiology · 2023Article
- RNA Interference of Chitin Synthase 2 Gene inInsects · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
- Stability of Nuclear and Mitochondrial Reference Genes in Selected Tissues of the Ambrosia BeetleInsects · 2021Article
- Identification of suitable reference genes for expression profiling studies using qRT-PCR in an important insect pest, Maruca vitrata.Molecular biology reports · 2021Article
- Assessing the effects of a novel biostimulant to enhance leafminer resistance and plant growth on common bean.Scientific reports · 2021Article
- High Incidence of RelatedInsects · 2021Article
- Transcriptional regulation of small heat shock protein genes by heat shock factor 1 (HSF1) in Liriomyza trifolii under heat stress.Cell stress & chaperones · 2021Article
- Article
- Selection and Validation of Reference Genes For qRT-PCR Analysis ofFrontiers in physiology · 2021Article
- Article
- Article
- Selection and Validation of Reference Genes for RT-qPCR Normalization inFrontiers in physiology · 2021Article
- Selection and validation of experimental condition-specific reference genes for qRT-PCR in Metopolophium dirhodum (Walker) (Hemiptera: Aphididae).Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liriomyza trifolii is a highly-invasive leafmining insect that causes significant damage to vegetables and horticultural crops worldwide. Relatively few studies have quantified gene expression in L. trifolii using real-time quantitative PCR (RT-qPCR), which is a reliable and sensitive technique for measuring gene expression. RT-qPCR requires the selection of reference genes to normalize gene expression data and control for internal differences between samples. In this study, nine housekeeping genes from L. trifolii were selected for their suitability in normalizing gene expression using geNorm, Normfinder, BestKeeper, the ΔCt method and RefFinder. HSP21.7, which encodes heat shock protein 21.7, was used as a target gene to validate the expression of candidate reference genes. Results indicated that ACTIN and 18S were optimal for developmental stage and low temperature, TUB and 18S showed the most stable expression for sex, and GAPDH and ACTIN were the best reference genes for monitoring gene expression at high temperature. Selection and validation of appropriate reference genes are critical steps in normalizing gene expression levels, which improve the accuracy and quality of expression data. Results of this study provide vital information on reference genes and is valuable in developing a standardized RT-qPCR protocol for functional genomics research in L. trifolii.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.