ArticleNature2017
Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation.
Article in Nature, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
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Who cites it
118 citing papers in PubMed, 213 citations in OpenAlex.
- Overconfluent culture promotes cell cycle synchronization in mouse embryonic stem cells for efficient cloned embryo production.The Journal of reproduction and development · 2026Article
- A panel of Diversity Outbred mouse embryonic stem cells: enabling the functional characterization of natural genetic variationbioRxiv : the preprint server for biology · 2026Article
- Rat cell-derived kidney generation via interspecies blastocyst complementation in an Osr1-KO mouse model.Stem cell reports · 2026Article
- PCBP1 orchestrates amino acid metabolism burst during the naïve-to-primed pluripotency transition.Stem cell reports · 2026Article
- Mechanisms coordinating exit from the stem cell state in mammals.Genes & development · 2026Review
- Uncovering the acetylation sites of Dnmt3L that regulate protein stability and differentiation potency in embryonic stem cells.Experimental & molecular medicine · 2026Article
- Depletion of Sorcs3 Activates Totipotency in Mouse Embryonic Stem Cells by Modulating Key Signaling Pathways.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cdh2, a downstream target of Hes7, regulates somitogenesis by supporting FGF signalling.Development (Cambridge, England) · 2025Article
- Why are imprints unstable in pluripotent stem cells?Biochemical Society transactions · 2025Article
- Article
- Stem cell-based embryo models: a tool to study early human development.Science China. Life sciences · 2025Review
- Totipotency or plenipotency: rethinking stem cell bipotentiality.Current opinion in genetics & development · 2025Review
- Efficient generation of all ESC-derived mice carrying a homozygous lethal mutation through eight-cell embryo injection.Science China. Life sciences · 2025Article
- The building blocks of embryo models: embryonic and extraembryonic stem cells.Cell discovery · 2025Review
- Enhancing the neural differentiation capabilities of genetically asymmetric mouse F1 hybrid embryonic stem cell lines.Experimental animals · 2025Article
- Genetic variation modulates susceptibility to aberrant DNA hypomethylation and imprint deregulation in naive pluripotent stem cells.Stem cell reports · 2025Article
- Capture of Totipotency in Mouse Embryonic Stem Cells in the Absence of Pdzk1.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Generation of live mice from haploid ESCs with germline-DMR deletions or switch.Cell discovery · 2025Article
- Glycosylation in Stem Cell Biology.Handbook of experimental pharmacology · 2025Review
- Blastocyst complementation-based rat-derived heart generation reveals cardiac anomaly barriers to interspecies chimera development.iScience · 2024Article
58 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inhibitors of Mek1/2 and Gsk3β, known as 2i, enhance the derivation of embryonic stem (ES) cells and promote ground-state pluripotency in rodents. Here we show that the derivation of female mouse ES cells in the presence of 2i and leukaemia inhibitory factor (2i/L ES cells) results in a widespread loss of DNA methylation, including a massive erasure of genomic imprints. Despite this global loss of DNA methylation, early-passage 2i/L ES cells efficiently differentiate into somatic cells, and this process requires genome-wide de novo DNA methylation. However, the majority of imprinting control regions (ICRs) remain unmethylated in 2i/L-ES-cell-derived differentiated cells. Consistently, 2i/L ES cells exhibit impaired autonomous embryonic and placental development by tetraploid embryo complementation or nuclear transplantation. We identified the derivation conditions of female ES cells that display 2i/L-ES-cell-like transcriptional signatures while preserving gamete-derived DNA methylation and autonomous developmental potential. Upon prolonged culture, however, female ES cells exhibited ICR demethylation regardless of culture conditions. Our results provide insights into the derivation of female ES cells reminiscent of the inner cell mass of preimplantation embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.