ArticleThe Analyst2017
Charged poly(N-isopropylacrylamide) nanogels for use as differential protein receptors in a turbidimetric sensor array.
Article in The Analyst, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Temperature-Sensitive Sensors Modified with Poly(N-isopropylacrylamide): Enhancing Performance through Tailored Thermoresponsiveness.Molecules (Basel, Switzerland) · 2024Article
- Protein-Nanoparticle Interactions Govern the Interfacial Behavior of Polymeric Nanogels: Study of Protein Corona Formation at the Air/Water Interface.International journal of molecular sciences · 2023Article
- Water as the often neglected medium at the interface between materials and biology.Nature communications · 2022Review
- Advanced biomedical hydrogels: molecular architecture and its impact on medical applications.Regenerative biomaterials · 2021Review
- Electrostatic and Covalent Assemblies of Anionic Hydrogel-Coated Gold Nanoshells for Detection of Dry Eye Biomarkers in Human Tears.Nano letters · 2021Article
- Charged Poly(ACS biomaterials science & engineering · 2021Article
- Recent Advancements in Biosensing Approaches for Screening and Diagnostic Applications.Current opinion in biomedical engineering · 2021Article
- Peptide conjugation enhances the cellular co-localization, but not endosomal escape, of modular poly(acrylamide-co-methacrylic acid) nanogels.Journal of controlled release : official journal of the Controlled Release Society · 2021Article
- Nanogel receptors for high isoelectric point protein detection: influence of electrostatic and covalent polymer-protein interactions.Chemical communications (Cambridge, England) · 2020Article
- Molecular recognition with soft biomaterials.Soft matter · 2020Article
- Modular Fabrication of Intelligent Material-Tissue Interfaces for Bioinspired and Biomimetic Devices.Progress in materials science · 2019Article
- Label-Free Detection of Tear Biomarkers Using Hydrogel-Coated Gold Nanoshells in a Localized Surface Plasmon Resonance-Based Biosensor.ACS nano · 2018Article
- Functional Stimuli-Responsive Gels: Hydrogels and Microgels.Gels (Basel, Switzerland) · 2018Review
- Tunable poly(methacrylic acid-co-acrylamide) nanoparticles through inverse emulsion polymerization.Journal of biomedical materials research. Part A · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Due to the high cost and environmental instability of antibodies, there is precedent for developing synthetic molecular recognition agents for use in diagnostic sensors. While these materials typically have lower specificity than antibodies, their cross-reactivity makes them excellent candidates for use in differential sensing routines. In the current work, we design a set of charge-containing poly(N-isopropylacrylamide) (PNIPAM) nanogels for use as differential protein receptors in a turbidimetric sensor array. Specifically, NIPAM was copolymerized with methacrylic acid and modified via carbodiimide coupling to introduce sulfate, guanidinium, secondary amine, or primary amine groups. Modification of the ionizable groups in the network changed the physicochemical and protein binding properties of the nanogels. For high affinity protein-polymer interactions, turbidity of the nanogel solution increased, while for low affinity interactions minimal change in turbidity was observed. Thus, relative turbidity was used as input for multivariate analysis. Turbidimetric assays were performed in two buffers of different pH (i.e., 7.4 and 5.5), but comparable ionic strength, in order to improve differentiation. Using both buffers, it was possible to achieve 100% classification accuracy of eleven model protein biomarkers with as few as two of the nanogel receptors. Additionally, it was possible to detect changes in lysozyme concentration in a simulated tear fluid using the turbidimetric sensor array.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.