ArticleJournal of visualized experiments : JoVE2017
Determination of the Glycogen Content in Cyanobacteria.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- High-Throughput Screening of Cyanobacterial PHB Production Using Fluorescence-Based Detection.Microbial biotechnology · 2026Article
- Synergistic Effects of Nitrogen Application Enhance Drought Resistance inPlants (Basel, Switzerland) · 2025Article
- An Open-Source Modular Bioreactor Platform for Cultivation of Synechocystis sp. PCC 6803 and Extraction of Intracellular Glucose.Processes (Basel, Switzerland) · 2025Article
- Phosphoketolase and KDPG aldolase metabolisms modulate photosynthetic carbon yield in cyanobacteria.The Plant cell · 2024Article
- Cyanobacterial Algal Bloom Monitoring: Molecular Methods and Technologies for Freshwater Ecosystems.Microorganisms · 2023Review
- Revalorization of Microalgae Biomass for Synergistic Interaction and Sustainable Applications: Bioplastic Generation.Marine drugs · 2022Review
- Thermodynamics contributes to high limonene productivity in cyanobacteria.Metabolic engineering communications · 2022Article
- Generation of miniploid cells and improved natural transformation procedure for a model cyanobacteriumFrontiers in microbiology · 2022Article
- Combining metabolite doping and metabolic engineering to improve 2-phenylethanol production by engineered cyanobacteria.Frontiers in bioengineering and biotechnology · 2022Article
- Over Expression of the Cyanobacterial Pgr5-Homologue Leads to Pseudoreversion in a Gene Coding for a Putative Esterase in Synechocystis 6803.Life (Basel, Switzerland) · 2020Article
- Bioethanol production from microalgae polysaccharides.Folia microbiologica · 2019Review
- Biophotovoltaics: Green Power Generation From Sunlight and Water.Frontiers in microbiology · 2019Review
- Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method.Bio-protocol · 2018Article
- Easy and Efficient Permeabilization of Cyanobacteria forBio-protocol · 2018Article
- Chronic Physical Disturbance Substantially Alters the Response of Biological Soil Crusts to a Wetting Pulse, as Characterized by Metatranscriptomic Sequencing.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria accumulate glycogen as a major intracellular carbon and energy storage during photosynthesis. Recent developments in research have highlighted complex mechanisms of glycogen metabolism, including the diel cycle of biosynthesis and catabolism, redox regulation, and the involvement of non-coding RNA. At the same time, efforts are being made to redirect carbon from glycogen to desirable products in genetically engineered cyanobacteria to enhance product yields. Several methods are used to determine the glycogen contents in cyanobacteria, with variable accuracies and technical complexities. Here, we provide a detailed protocol for the reliable determination of the glycogen content in cyanobacteria that can be performed in a standard life science laboratory. The protocol entails the selective precipitation of glycogen from the cell lysate and the enzymatic depolymerization of glycogen to generate glucose monomers, which are detected by a glucose oxidase-peroxidase (GOD-POD) enzyme coupled assay. The method has been applied to Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002, two model cyanobacterial species that are widely used in metabolic engineering. Moreover, the method successfully showed differences in the glycogen contents between the wildtype and mutants defective in regulatory elements or glycogen biosynthetic genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.