ArticleJournal of visualized experiments : JoVE2017
Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- Late gestational exposure to flutamide alters stromal composition and immune landscape in the rat mammary gland during pre-puberty, peri-puberty, and adulthood.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- An in utero exposure to the synthetic estrogen diethylstilbestrol affects the fat pad composition in post-natal mammary glands.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- In utero exposure to estrogenic bisphenol analogues increases mammary tissue stiffness.Reproductive toxicology (Elmsford, N.Y.) · 2025Article
- DEK promotes mammary hyperplasia and is associated with H3K27me3 epigenetic modifications.Life science alliance · 2025Article
- The rat mammary gland undergoes dynamic transcriptomic and lipidomic modifications from pre-puberty to adulthood.Scientific reports · 2025Article
- Hypoxic Preconditioning Mitigates Acute Hypoxia Induced MS/VDB Cholinergic Cell Loss and Memory Impairments.Aging and disease · 2025Article
- Microvascular Regeneration and Perfusion of Partially Decellularized Tracheal Grafts.The Laryngoscope · 2025Article
- Whole mount preparation and analysis of rabbit mammary gland.Reproductive toxicology (Elmsford, N.Y.) · 2024Article
- GOS enhances BDNF-mediated mammary gland development in pubertal mice via the gut-brain axis.NPJ biofilms and microbiomes · 2024Article
- Mammary gland development and EDC-driven cancer susceptibility in mesenchymal ERα-knockout mice.Endocrine-related cancer · 2023Article
- Single neonatal estrogen implant sterilizes female animals by decreasing hypothalamic KISS1 expression.Scientific reports · 2023Article
- Effect of Maternal Gradient Nutritional Restriction during Pregnancy on Mammary Gland Development in Offspring.Animals : an open access journal from MDPI · 2023Article
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- Phosphotungstic acid-enhanced microcomputed tomography for quantitative visualization of mouse mammary gland morphology.Journal of medical imaging (Bellingham, Wash.) · 2023Article
- Best practices to quantify the impact of reproductive toxicants on development, function, and diseases of the rodent mammary gland.Reproductive toxicology (Elmsford, N.Y.) · 2022Review
- Dynamic miRNA Landscape Links Mammary Gland Development to the Regulation of Milk Protein Expression in Mice.Animals : an open access journal from MDPI · 2022Article
- Prolactin synergizes with canonical Wnt signals to drive development of ER+ mammary tumors via activation of the Notch pathway.Cancer letters · 2021Article
- Systemic alterations play a dominant role in epigenetic predisposition to breast cancer in offspring of obese fathers and is transmitted to a second generation.Scientific reports · 2021Article
- Mutant SF3B1 promotes AKT- and NF-κB-driven mammary tumorigenesis.The Journal of clinical investigation · 2021Article
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Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
An increasing number of studies are utilizing the rodent mammary gland as an endpoint for assessing the developmental toxicity of a chemical exposure. The effects these exposures have on mammary gland development are typically evaluated using either basic dimensional measurements or by scoring morphological characteristics. However, the broad range of methods for interpreting developmental changes could lead to inconsistent translations across laboratories. A common method of assessment is needed so that proper interpretations can be formed from data being compared across studies. The present study describes the application of the Sholl analysis method to quantify mammary gland branching characteristics. The Sholl method was originally developed for use in quantifying neuronal dendritic patterns. By using ImageJ, an open-source image analysis software package, and a plugin developed for this analysis, the mammary gland branching density and the complexity of a mammary gland from a peripubertal female rat were determined. The methods described here will enable the use of the Sholl analysis as an effective tool for quantifying an important characteristic of mammary gland development.
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