ArticleProceedings of the National Academy of Sciences of the United States of America1986
In situ hybridization to localize mRNA encoding the neurotransmitter synthetic enzyme glutamate decarboxylase in mouse cerebellum.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 96 citations in OpenAlex.
- The Cerebellar Dopaminergic System.Frontiers in systems neuroscience · 2021Review
- Impaired neurogenesis, learning and memory and low seizure threshold associated with loss of neural precursor cell survivin.BMC neuroscience · 2010Article
- Decreased GAD65 mRNA levels in select subpopulations of neurons in the cerebellar dentate nuclei in autism: an in situ hybridization study.Autism research : official journal of the International Society for Autism Research · 2009Article
- The neurochemically diverse intermedius nucleus of the medulla as a source of excitatory and inhibitory synaptic input to the nucleus tractus solitarii.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- GABAergic neurons in the central region of the spinal cord: a novel substrate for sympathetic inhibition.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Localization and function of the Kv3.1b subunit in the rat medulla oblongata: focus on the nucleus tractus solitarii.The Journal of physiology · 2005Article
- Use-dependent modulation of inhibitory capacity in the feline lumbar spinal cord.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Presence of mRNA for glutamic acid decarboxylase in both excitatory and inhibitory neurons.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- The mismatch problem for GABAergic amacrine cells in goldfish retina: resolution and other issues.Neurochemical research · 1991Article
- The structural and functional heterogeneity of glutamic acid decarboxylase: a review.Neurochemical research · 1991Review
- Developmentally regulated expression of an exon containing a stop codon in the gene for glutamic acid decarboxylase.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Pattern of expression of glutamic acid decarboxylase mRNA in the developing rat brain.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Localization of mRNAs for the Small and Large Subunits of Rubisco Using Electron Microscopic in Situ Hybridization.Plant physiology · 1988Article
- Expression of the insulin-like growth factor II gene in the choroid plexus and the leptomeninges of the adult rat central nervous system.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Brain glutamate decarboxylase cloned in lambda gt-11: fusion protein produces gamma-aminobutyric acid.Science (New York, N.Y.) · 1986Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Glutamate decarboxylase (GAD; EC 4.1.1.15) is responsible for the synthesis of the neurotransmitter gamma-aminobutyric acid (GABA). We have used a cDNA sequence encoding GAD to produce a single-stranded RNA hybridization probe for GAD mRNA. This probe detects GAD mRNA in individual cells in sections of mouse cerebellum. The specificity of in situ hybridization with this probe rests on four criteria: the distribution of labeled cells matched the results we and others obtain with GAD immunohistochemistry (Purkinje, Golgi II, stellate, and basket neurons were labeled, whereas granule cells and glia were not); a negative control probe having a sequence identical to GAD mRNA did not specifically label any cerebellar cells; prior treatment of the sections with RNase abolished specific labeling; the labeling showed the melting behavior typical of nucleic acid hybrids. Translation of GAD mRNA is apparently restricted to neuronal cell bodies since GAD mRNA was detectable in neuronal perikarya but not in terminals. Also, the choice of GABA as a neurotransmitter appears to be made at the level of transcription since granule neurons did not contain detectable GAD mRNA. The level of GAD mRNA varied among the classes of neurons as well as from cell to cell within each neuron type.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.