Evidence map›Paper›PMID 28745544›Full record

ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2017

Euptox A Induces G1 Arrest and Autophagy via p38 MAPK- and PI3K/Akt/mTOR-Mediated Pathways in Mouse Splenocytes.

Quan Mo, Liwen Hu, Jiahua Weng, Yong Zhang, Yancheng Zhou, Ruiguang Xu, Zhicai Zuo, Junliang Deng, Zhihua Ren, Zhijun Zhong and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.3field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
  3. Equine Crofton Weed (Animals : an open access journal from MDPI · 2023
    Review
  4. Article
  5. Frontiers in microbiology · 2022
    Article
  6. An Overview: The Toxicity ofInternational journal of molecular sciences · 2021
    Review
  7. Toxins · 2021
    Article
  8. Toxins · 2021
    Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Quan MoKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Liwen HuKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Jiahua WengKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Yong ZhangKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Yancheng ZhouKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Ruiguang XuKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Zhicai ZuoKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Junliang DengKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Zhihua RenKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Zhijun ZhongKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Guangneng PengKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Xiang NongCollege of Life Science, Leshan Normal University, Leshan, China (XN).
Yahui WeiKey Laboratory of Resource Biology and Biotechnology in Western China, School of Life Science, Northwest University, Xi'an, China (YW).
Yanchun HuKey laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China (QM, LH, JW, YZ, YCZ, RX, ZCZ, JD, ZR, ZJZ, GP, YH).
Sichuan Agricultural University · CNLeshan Normal University · CNNorthwest University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Euptox A (9-oxo-10, 11-dehydroageraphorone), the main toxin isolated from Eupatorium adenophorum, is known to induce immunotoxicity in animals. However, the precise mechanism underlying the effects of Euptox A on splenocytes is unclear. Here, we aimed to investigate the molecular mechanisms underlying the effect of Euptox A in mouse spleens after its intragastric administration and found that Euptox A exhibits proautophagic effects in splenocytes. Euptox A markedly arrested the splenocytes in the G0/G1 phase, which was accompanied by inhibition of the expression of the positive regulators CDK4, CDK2, cyclin D1, PCNA, and E2F1, and promotion of the expression of the negative regulators p53, p21 Waf1/Cip1, p27 Kip1, and Chk1. We also found that Euptox A did not markedly induce splenocyte apoptosis, but induced autophagy while increasing the subcellular localization of punctate LC3, ratio of LC3-II/LC3-I, and Beclin 1 levels, and decreasing p62 levels. Euptox A also significantly inhibited p-PI3K, p-p38 MAPK, p-Akt, and p-mTOR expression, but increased PTEN and p-AMPK expression. These results indicated that Euptox A induced splenocyte autophagy by inhibiting the PI3K/Akt/mTOR pathway, suppressing p38 MAPK expression, and activating AMPK. These findings provide new insights into the mechanisms involved in spleen toxicity caused by Euptox A in mice.

Indexed as

AgeratinaAnimalsApoptosisAutophagyDose-Response Relationship, DrugG1 Phase Cell Cycle CheckpointsMembrane Potential, MitochondrialMicep38 Mitogen-Activated Protein KinasesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSesquiterpenesSignal TransductionSpleenStructure-Activity RelationshipTOR Serine-Threonine Kinases9-oxo-10,11-dehydroagerophoronep38 Mitogen-Activated Protein KinasesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSesquiterpenesTOR Serine-Threonine Kinases11-dehydroageraphorone9-oxo-10apoptosisautophagycell cycle arrestE. adenophorumPI3K/Akt/mTOR pathway

Identifiers

PMID28745544
PMCPMC5582668
OpenAlexW2737095191

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.