ArticleCellular microbiology2017
Distinct roles of the 7-transmembrane receptor protein Rta3 in regulating the asymmetric distribution of phosphatidylcholine across the plasma membrane and biofilm formation in Candida albicans.
Article in Cellular microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Miltefosine regulates gene expression related to morphogenesis and zinc metabolism inMicrobiology spectrum · 2026Article
- RTA-like proteins regulate azole susceptibility by affecting the expression of the oxidoreductase gene oxrA in Aspergillus fumigatus.BMC microbiology · 2026Article
- Lem3, a phospholipid flippase subunit, influencesMicrobiology spectrum · 2026Article
- Rta1 lipid transporter involved in aluminum and acid tolerance in Cryptococcus humicola.Folia microbiologica · 2025Article
- Proximity labeling identification of plasma membrane eisosome proteins in Candida albicans.Genetics · 2025Article
- Biosynthesis of the Antifungal Polyhydroxy-Polyketide Acrophialocinol.Organic letters · 2025Article
- The zinc cluster transcription factor Znc1 regulates Rta3-dependent miltefosine resistance inmSphere · 2024Article
- Fungal biofilm formation and its regulatory mechanism.Heliyon · 2024Review
- Article
- Characterization of theJournal of fungi (Basel, Switzerland) · 2022Article
- Regulatory network controls microbial biofilm development, withBioengineered · 2022Review
- Ifu5, a WW domain-containing protein interacts with Efg1 to achieve coordination of normoxic and hypoxic functions to influence pathogenicity traits in Candida albicans.Cellular microbiology · 2020Article
- Disruption of Membrane Integrity by the Bacterium-Derived Antifungal Jagaricin.Antimicrobial agents and chemotherapy · 2019Article
- Role of lipid transporters in fungal physiology and pathogenicity.Computational and structural biotechnology journal · 2019Review
- The Significance of Lipids to Biofilm Formation inJournal of fungi (Basel, Switzerland) · 2018Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Fungal pathogens such as Candida albicans exhibit several survival mechanisms to evade attack by antifungals and colonise host tissues. Rta3, a member of the Rta1-like family of lipid-translocating exporters has a 7-transmembrane domain topology, similar to the G-protein-coupled receptors and is unique to the fungal kingdom. Our findings point towards a role for the plasma membrane localised Rta3 in providing tolerance to miltefosine, an analogue of alkylphosphocholine, by maintaining mitochondrial energetics. Concurrent with miltefosine susceptibility, the rta3Δ/Δ strain displays increased inward translocation (flip) of fluorophore-labelled phosphatidylcholine (PC) across the plasma membrane attributed to enhanced PC-specific flippase activity. We also assign a novel role to Rta3 in the Bcr1-regulated pathway for in vivo biofilm development. Transcriptome analysis reveals that Rta3 regulates expression of Bcr1 target genes involved in cell surface properties, adhesion, and hyphal growth. We show that rta3Δ/Δ mutant is biofilm-defective in a rat venous catheter model of infection and that BCR1 overexpression rescues this defect, indicating that Bcr1 functions downstream of Rta3 to mediate biofilm formation in C. albicans. The identification of this novel Rta3-dependent regulatory network that governs biofilm formation and PC asymmetry across the plasma membrane will provide important insights into C. albicans pathogenesis.
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