ArticleExtremophiles : life under extreme conditions2017
Cloning, overexpression, and characterization of a thermostable nitrilase from an Antarctic Pyrococcus sp.
Article in Extremophiles : life under extreme conditions, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 23 citations in OpenAlex.
- Recombinant expression and characterization of a new laccase, bioinformatically identified, from the Antarctic thermophilic bacterium Geobacillus sp. ID17.Extremophiles : life under extreme conditions · 2023Article
- Comparative Genomic Analysis of AntarcticGenes · 2022Article
- Purification and Characterization of NitFrontiers in bioengineering and biotechnology · 2021Article
- Biotechnological applications of archaeal enzymes from extreme environments.Biological research · 2018Review
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2 authors at 2 institutions in 1 country.
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Abstract
Nitriles are important chemical building blocks for the synthesis of intermediates in fine chemical and pharmaceutical industries. Here, we report a new highly thermostable nitrilase from an Antarctic Pyrococcus sp. MC-FB, a hyperthermophilic archaeon. A gene that encoded a nitrilase was identified and subsequently cloned and overexpressed in Escherichia coli. The recombinant nitrilase, named NitMC-FB, is active as a homodimer (60 kDa) with an optimal temperature and pH of 90 °C and 7.0, respectively. NitMC-FB hydrolyzes preferentially aromatic nitriles, being the first aromatic nitrilase from an archaeon described so far. The K
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