Evidence map›Paper›PMID 28744331›Full record

ArticleTheranostics2017

Fabrication of Tissue-Engineered Bionic Urethra Using Cell Sheet Technology and Labeling By Ultrasmall Superparamagnetic Iron Oxide for Full-Thickness Urethral Reconstruction.

Shukui Zhou, Ranxin Yang, Qingsong Zou, Kaile Zhang, Ting Yin, Weixin Zhao, Joseph G Shapter, Guo Gao, Qiang Fu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Theranostics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
6.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.

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  19. Theranostic biomaterials for tissue engineering.Current opinion in biomedical engineering · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

Shukui ZhouDepartment of Urology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Ranxin YangDepartment of Urology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Qingsong ZouDepartment of Urology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Kaile ZhangDepartment of Urology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Ting YinInstitute of Nano Biomedicine and Engineering, Department of Instrument Science and Technology, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Weixin ZhaoWake Forest Institute for Regenerative Medicine, Winston Salem, NC, USA.
Joseph G ShapterSchool of Chemical and Physical Sciences, Flinders University, Bedford Park, Adelaide 5042, Australia.
Guo GaoInstitute of Nano Biomedicine and Engineering, Department of Instrument Science and Technology, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Qiang FuDepartment of Urology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Jiao Tong University · CNShanghai Sixth People's Hospital · CNFlinders University · AUForest Institute · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Urethral strictures remain a reconstructive challenge, due to less than satisfactory outcomes and high incidence of stricture recurrence. An "ideal" urethral reconstruction should establish similar architecture and function as the original urethral wall. We fabricated a novel tissue-engineered bionic urethras using cell sheet technology and report their viability in a canine model. Small amounts of oral and adipose tissues were harvested, and adipose-derived stem cells, oral mucosal epithelial cells, and oral mucosal fibroblasts were isolated and used to prepare cell sheets. The cell sheets were hierarchically tubularized to form 3-layer tissue-engineered urethras and labeled by ultrasmall super-paramagnetic iron oxide (USPIO). The constructed tissue-engineered urethras were transplanted subcutaneously for 3 weeks to promote the revascularization and biomechanical strength of the implant. Then, 2 cm length of the tubularized penile urethra was replaced by tissue-engineered bionic urethra. At 3 months of urethral replacement, USPIO-labeled tissue-engineered bionic urethra can be effectively detected by MRI at the transplant site. Histologically, the retrieved bionic urethras still displayed 3 layers, including an epithelial layer, a fibrous layer, and a myoblast layer. Three weeks after subcutaneous transplantation, immunofluorescence analysis showed the density of blood vessels in bionic urethra was significantly increased following the initial establishment of the constructs and was further up-regulated at 3 months after urethral replacement and was close to normal level in urethral tissue. Our study is the first to experimentally demonstrate 3-layer tissue-engineered urethras can be established using cell sheet technology and can promote the regeneration of structural and functional urethras similar to normal urethra.

Indexed as

Organ Culture TechniquesAnimalsBionicsContrast MediaDextransDogsEpithelial CellsFibroblastsFluorescent Antibody TechniqueMagnetic Resonance ImagingMagnetite NanoparticlesStaining and LabelingStem CellsTissue EngineeringTransplantsUrethraContrast MediaDextransferumoxtran-10Magnetite Nanoparticlesbionic urethracell sheet technologytissue engineeringtransplant imagingultrasmall super-paramagnetic iron oxide (USPIO).urethral reconstruction

Identifiers

PMID28744331
PMCPMC5525753
OpenAlexW2734678427

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.