ArticleScientific reports2017
A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Article
- An automated portable LAMP-based centrifugal microfluidic system for nucleic acid detection of multiple pathogens in feline upper respiratory disease.Mikrochimica acta · 2025Article
- AI-Enabled Microfluidics for Respiratory Pathogen Detection.Sensors (Basel, Switzerland) · 2025Review
- Rapid, season-specific PCR testing versus traditional diagnostics for pneumonia in the emergency department.BMC pulmonary medicine · 2025Article
- Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction.Journal of nanobiotechnology · 2025Article
- Detecting antibiotic resistance: classical, molecular, advanced bioengineering, and AI-enhanced approaches.Frontiers in microbiology · 2025Review
- A Diagnostic Chip for the Colorimetric Detection ofBiosensors · 2024Article
- Application of a clamshell isothermal nucleic acid amplification analyzer in the detection of lower respiratory tract bacteria.Practical laboratory medicine · 2024Article
- Rapid Detection ofJournal of microbiology and biotechnology · 2023Article
- An Integrated and Multi-Target Nucleic Acid Isothermal Analysis System for Rapid Diagnosis of Vulvovaginal Candidiasis.Biosensors · 2023Article
- Managing Viral Emerging Infectious Diseases via Current and Future Molecular Diagnostics.Diagnostics (Basel, Switzerland) · 2023Review
- Rapid, ultrasensitive and highly specific diagnosis ofFrontiers in cellular and infection microbiology · 2023Article
- Development of Microfluidic Chip-Based Loop-Mediated Isothermal Amplification (LAMP) Method for Detection of Carbapenemase Producing Bacteria.Microbiology spectrum · 2022Article
- Recent Progress in Spectroscopic Methods for the Detection of Foodborne Pathogenic Bacteria.Biosensors · 2022Review
- Fluorescent Biosensors for the Detection of Viruses Using Graphene and Two-Dimensional Carbon Nanomaterials.Biosensors · 2022Review
- Performance Evaluation of a Novel Ultrafast Molecular Diagnostic Device Integrated With Microfluidic Chips and Dual Temperature Modules.Frontiers in bioengineering and biotechnology · 2022Article
- Microfluidic Chip with Two-Stage Isothermal Amplification Method for Highly Sensitive Parallel Detection of SARS-CoV-2 and Measles Virus.Micromachines · 2021Article
- A Highly Automated Mobile Laboratory for On-site Molecular Diagnostics in the COVID-19 Pandemic.Clinical chemistry · 2021Article
- A fully automated microfluidic PCR-array system for rapid detection of multiple respiratory tract infection pathogens.Analytical and bioanalytical chemistry · 2021Article
- Review on the contamination of wastewater by COVID-19 virus: Impact and treatment.The Science of the total environment · 2021Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
An air-insulated microfluidic chip was designed for the automatic centrifugal distribution of samples to 24-test cells, enabling the parallel identification of multiple clinical pneumonia-related pathogens in 1.45-μL reactions without cross-contamination in 45 min. A portable nucleic acid analyzer that integrates mechanical, confocal optical, electronic, and software functions was also developed to collect fluorescence data in a Ø3 mm imaging field near the optical diffraction limit for highly sensitive fluorescence detection of nucleic acid amplification in real time. This microfluidic chip-based portable nucleic acid analyzer could detect low abundance nucleic acids present at as few as 10 copies. In a blinded experiment, specific identification of Mycoplasma pneumoniae, Staphylococcus aureus, and methicillin-resistant S. aureus was achieved with 229 clinical patient sputum samples. The total coincidence rate of our system and traditional RT-PCR with an ABI 7500 was 99.56%. Four samples accounting for the 0.44% inconformity were retested by gene sequencing, revealing that our system reported the correct results. This novel microfluidic chip-based detection system is cost-effective, rapid, sensitive, specific, and has a relatively high throughput for parallel identification, which is especially suitable for resource-limited facilities/areas and point-of-care testing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.