Evidence map›Paper›PMID 28743269›Full record

ArticleJournal of translational medicine2017

Mesenchymal stem cells from sternum: the type of heart disease, ischemic or valvular, does not influence the cell culture establishment and growth kinetics.

Lucinara Dadda Dias, Karina Rabello Casali, Carine Ghem, Melissa Kristocheck da Silva, Grasiele Sausen, Patrícia Bonini Palma, Dimas Tadeu Covas, Renato A K Kalil, Beatriz D Schaan, Nance Beyer Nardi and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of translational medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 6 institutions in 1 country.

Lucinara Dadda DiasPrograma de Pós-graduação em Ciências da Saúde-Cardiologia, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Avenida Princesa Isabel, n° 370, 3° andar, Porto Alegre, RS, CEP: 90620-001, Brazil.
Karina Rabello CasaliUniversidade Federal de São Paulo, São José dos Campos, SP, Brazil.
Carine GhemServiço de Patologia Clínica, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil.
Melissa Kristocheck da SilvaPrograma de Pós-graduação em Ciências da Saúde-Cardiologia, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Avenida Princesa Isabel, n° 370, 3° andar, Porto Alegre, RS, CEP: 90620-001, Brazil.
Grasiele SausenPrograma de Pós-graduação em Ciências da Saúde-Cardiologia, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Avenida Princesa Isabel, n° 370, 3° andar, Porto Alegre, RS, CEP: 90620-001, Brazil.
Patrícia Bonini PalmaLaboratório de Citometria de Fluxo, Centro Regional de Hemoterapia do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto/Universidade de São Paulo, São Paulo, SP, Brazil.
Dimas Tadeu CovasLaboratório de Citometria de Fluxo, Centro Regional de Hemoterapia do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto/Universidade de São Paulo, São Paulo, SP, Brazil.
Renato A K KalilPrograma de Pós-graduação em Ciências da Saúde-Cardiologia, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Avenida Princesa Isabel, n° 370, 3° andar, Porto Alegre, RS, CEP: 90620-001, Brazil.
Beatriz D SchaanFaculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Nance Beyer NardiPrograma de Pós-graduação em Ciências da Saúde-Cardiologia, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Avenida Princesa Isabel, n° 370, 3° andar, Porto Alegre, RS, CEP: 90620-001, Brazil.
Melissa Medeiros MarkoskiPrograma de Pós-graduação em Ciências da Saúde-Cardiologia, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Avenida Princesa Isabel, n° 370, 3° andar, Porto Alegre, RS, CEP: 90620-001, Brazil. melissa.markoski@gmail.com.ORCID 0000-0003-2982-5850
Fundação Universitária de Cardiologia · BRHospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo · BRUniversidade Federal de Ciências da Saúde de Porto Alegre · BRHospital de Clínicas de Porto Alegre · BRUniversidade Federal de São Paulo · BRUniversidade Federal do Rio Grande do Sul · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn an attempt to increase the therapeutic potential for myocardial regeneration, there is a quest for new cell sources and types for cell therapy protocols. The pathophysiology of heart diseases may affect cellular characteristics and therapeutic results.

methodsTo study the proliferative and differentiation potential of mesenchymal stem cells (MSC), isolated from bone marrow (BM) of sternum, we made a comparative analysis between samples of patients with ischemic (IHD) or non-ischemic valvular (VHD) heart diseases. We included patients with IHD (n = 42) or VHD (n = 20), with average age of 60 years and no differences in cardiovascular risk factors. BM samples were collected (16.4 ± 6 mL) and submitted to centrifugation with Ficoll-Paque, yielding 4.5 ± 1.5 × 10

resultsMorphology, immunophenotype and differentiation ability had proven that the cultivated sternal BM cells had MSC features. The colony forming unit-fibroblast (CFU-F) frequency was similar between groups (p = 0.510), but VHD samples showed positive correlation to plated cells vs. CFU-F number (r = 0.499, p = 0.049). The MSC culture was established in 29% of collected samples, achieved passage 9, without significant difference in expansion kinetics between groups (p > 0.05). Dyslipidemia and the use of statins was associated with culture establishment for IHD patients (p = 0.049 and p = 0.006, respectively).

conclusionsTogether, these results show that the sternum bone can be used as a source for MSC isolation, and that ischemic or valvular diseases do not influence the cellular yield, culture establishment or in vitro growth kinetics.

Indexed as

AgedCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedCell SeparationCell ShapeColony-Forming Units AssayFemaleHeart Valve DiseasesHumansImmunophenotypingKineticsMaleMesenchymal Stem CellsMiddle AgedCell therapyIschemic heart diseaseMSC establishmentStem cells cultureValvular heart disease

Identifiers

PMID28743269
PMCPMC5526254
OpenAlexW2737872417

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.