ArticlePLoS genetics2017
T-DNA-genome junctions form early after infection and are influenced by the chromatin state of the host genome.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Rooting Conifer Genetic Research: An Accessible and Efficient Transformation System.Plant biotechnology journal · 2026Article
- TONSOKU prevents the formation of large tandem duplications and restrains ATR-WEE1 checkpoint activation.Nature communications · 2026Article
- T-DNA orientation, distance between two T-DNAs, and the transformation target cells significantly impact vector backbone integration and efficiency of generating marker-free transgenic plants in a co-transformation system.The Plant journal : for cell and molecular biology · 2025Article
- Beyond a few bases: methods for large DNA insertion and gene targeting in plants.The Plant journal : for cell and molecular biology · 2025Review
- Arabidopsis F-box proteins D5BF1 and D5BF2 negatively regulate Agrobacterium-mediated transformation and tumorigenesis.Molecular plant pathology · 2024Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- Characterization of integration sites and transfer DNA structures in Agrobacterium-mediated transgenic events of maize inbred B104.G3 (Bethesda, Md.) · 2023Article
- SEGS-1 a cassava genomic sequence increases the severity of African cassava mosaic virus infection inFrontiers in plant science · 2023Article
- Identification of T-DNA structure and insertion site in transgenic crops using targeted capture sequencing.Frontiers in plant science · 2023Article
- Agrobacterium-mediated gene transfer: recent advancements and layered immunity in plants.Planta · 2022Review
- Distinct mechanisms for genomic attachment of the 5' and 3' ends of Agrobacterium T-DNA in plants.Nature plants · 2022Article
- Increased Agrobacterium-mediated transformation of Saccharomyces cerevisiae after deletion of the yeast ADA2 gene.Letters in applied microbiology · 2022Article
- Genetic factors governing bacterial virulence and host plant susceptibility duringAdvances in genetics · 2022Review
- Plant DNA Repair andInternational journal of molecular sciences · 2021Review
- Preculture in an enriched nutrient medium greatly enhances thePlant biotechnology (Tokyo, Japan) · 2021Article
- Agrobacterium T-DNA integration in somatic cells does not require the activity of DNA polymerase θ.The New phytologist · 2021Article
- De novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.PloS one · 2021Article
- Strategies to produce T-DNA free CRISPRed fruit trees via Agrobacterium tumefaciens stable gene transfer.Scientific reports · 2020Article
- Generation ofMicroorganisms · 2019Article
- Single Molecule Imaging of T-DNA Intermediates FollowingInternational journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens mediated T-DNA integration is a common tool for plant genome manipulation. However, there is controversy regarding whether T-DNA integration is biased towards genes or randomly distributed throughout the genome. In order to address this question, we performed high-throughput mapping of T-DNA-genome junctions obtained in the absence of selection at several time points after infection. T-DNA-genome junctions were detected as early as 6 hours post-infection. T-DNA distribution was apparently uniform throughout the chromosomes, yet local biases toward AT-rich motifs and T-DNA border sequence micro-homology were detected. Analysis of the epigenetic landscape of previously isolated sites of T-DNA integration in Kanamycin-selected transgenic plants showed an association with extremely low methylation and nucleosome occupancy. Conversely, non-selected junctions from this study showed no correlation with methylation and had chromatin marks, such as high nucleosome occupancy and high H3K27me3, that correspond to three-dimensional-interacting heterochromatin islands embedded within euchromatin. Such structures may play a role in capturing and silencing invading T-DNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.