Evidence map›Paper›PMID 28741386›Full record

ArticleJournal of enzyme inhibition and medicinal chemistry2017

Cloning, expression and purification of the α-carbonic anhydrase from the mantle of the Mediterranean mussel, Mytilus galloprovincialis.

Rosa Perfetto, Sonia Del Prete, Daniela Vullo, Vincenzo Carginale, Giovanni Sansone, Carmela M A Barone, Mosè Rossi, Fatmah A S Alasmary, Sameh M Osman, Zeid AlOthman and 2 more

Abstract read
In one paragraph

Article in Journal of enzyme inhibition and medicinal chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Carbonic Anhydrase in Pacific AbaloneFrontiers in molecular biosciences · 2021
    Article
  2. Review
  3. Article
  4. The first activation study of the β-carbonic anhydrases from the pathogenic bacteriaJournal of enzyme inhibition and medicinal chemistry · 2019
    Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Rosa Perfettoa Istituto di Bioscienze e Biorisorse , CNR , Napoli , Italy.
Sonia Del Pretea Istituto di Bioscienze e Biorisorse , CNR , Napoli , Italy.
Daniela Vullob Laboratorio di Chimica Bioinorganica, Polo Scientifico , Università degli Studi di Firenze , Sesto Fiorentino, Florence , Italy.
Vincenzo Carginalea Istituto di Bioscienze e Biorisorse , CNR , Napoli , Italy.
Giovanni Sansonec Dipartimento di Biologia , Università degli Studi di Napoli, Federico II , Napoli , Italy.
Carmela M A Baroned Dipartimento di Agraria , Università degli Studi di Napoli, Federico II , Portici, Napoli , Italy.
Mosè Rossia Istituto di Bioscienze e Biorisorse , CNR , Napoli , Italy.
Fatmah A S Alasmarye Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche , Università degli Studi di Firenze , Sesto Fiorentino , Florence , Italy.
Sameh M Osmane Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche , Università degli Studi di Firenze , Sesto Fiorentino , Florence , Italy.
Zeid AlOthmane Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche , Università degli Studi di Firenze , Sesto Fiorentino , Florence , Italy.
Claudiu T Supuranb Laboratorio di Chimica Bioinorganica, Polo Scientifico , Università degli Studi di Firenze , Sesto Fiorentino, Florence , Italy.
Clemente Capassoa Istituto di Bioscienze e Biorisorse , CNR , Napoli , Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We cloned, expressed, purified, and determined the kinetic constants of the recombinant α-carbonic anhydrase (rec-MgaCA) identified in the mantle tissue of the bivalve Mediterranean mussel, Mytilus galloprovincialis. In metazoans, the α-CA family is largely represented and plays a pivotal role in the deposition of calcium carbonate biominerals. Our results demonstrated that rec-MgaCA was a monomer with an apparent molecular weight of about 32 kDa. Moreover, the determined kinetic parameters for the CO

Indexed as

AnimalsCarbonic AnhydrasesCloning, MolecularMytilusCarbonic AnhydrasesbivalveCarbonic anhydrasehydratase activitymetalloenzymesmultidomain proteinmusselprotonographyα-class enzyme

Identifiers

PMID28741386
PMCPMC6010101

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.