ArticleTheranostics2017
A Paper-Based Device for Performing Loop-Mediated Isothermal Amplification with Real-Time Simultaneous Detection of Multiple DNA Targets.
Article in Theranostics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 147 citations in OpenAlex.
- Extraction-Free Scalable Sample Preparation for the Rapid Detection of HIV in Blood Plasma.Analytical chemistry · 2026Article
- Paper-Based Microfluidic Chips for At-Home Point-of-Care Nucleic Acid Testing: Applications and Challenges.Diagnostics (Basel, Switzerland) · 2026Review
- Article
- Fluorescence origami paper-based analytical device based on strand displacement assay for SARS-CoV-2 cDNA detection.Mikrochimica acta · 2025Article
- Molecular diagnostic methods for rapid diagnosis of central nervous system infections.Frontiers in medical technology · 2025Review
- Miniaturized electrophoresis: An integrated microfluidic cartridge with functionalized hydrogel-assisted LAMP for sample-to-answer analysis of nucleic acid.Biomicrofluidics · 2024Article
- Vibration mixing for enhanced paper-based recombinase polymerase amplification.Lab on a chip · 2024Article
- Article
- Article
- Method for lysis and paper-based elution-free DNA extraction with colourimetric isothermal amplification.Scientific reports · 2024Article
- Development and evaluation of a lyophilization protocol for colorimetric RT-LAMP diagnostic assay for COVID-19.Scientific reports · 2024Article
- Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes.Diagnostics (Basel, Switzerland) · 2024Article
- Diagnosis of extraintestinal pathogenicCurrent research in microbial sciences · 2024Review
- Review
- Trends of respiratory virus detection in point-of-care testing: A review.Analytica chimica acta · 2023Review
- Paper-based sensors for bacteria detection.Nature reviews bioengineering · 2023Review
- A portable system for economical nucleic acid amplification testing.Frontiers in bioengineering and biotechnology · 2023Article
- A Novel miRNA Detection Method Using Loop-Mediated Isothermal Amplification.International journal of analytical chemistry · 2023Article
- Review
- Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paper-based diagnostic devices have many advantages as a one of the multiple diagnostic test platforms for point-of-care (POC) testing because they have simplicity, portability, and cost-effectiveness. However, despite high sensitivity and specificity of nucleic acid testing (NAT), the development of NAT based on a paper platform has not progressed as much as the others because various specific conditions for nucleic acid amplification reactions such as pH, buffer components, and temperature, inhibitions from technical differences of paper-based device. Here, we propose a paper-based device for performing loop-mediated isothermal amplification (LAMP) with real-time simultaneous detection of multiple DNA targets. We determined the optimal chemical components to enable dry conditions for the LAMP reaction without lyophilization or other techniques. We also devised the simple paper device structure by sequentially stacking functional layers, and employed a newly discovered property of hydroxynaphthol blue fluorescence to analyze real-time LAMP signals in the paper device. This proposed platform allowed analysis of three different meningitis DNA samples in a single device with single-step operation. This LAMP-based multiple diagnostic device has potential for real-time analysis with quantitative detection of 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.