ArticleScientific reports2017
Inactivation Mechanism of Escherichia coli Induced by Slightly Acidic Electrolyzed Water.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 56 citations in OpenAlex.
- Electrolyzed saline as an alternative to chlorhexidine: Antimicrobial and biofilm volume outcomes in a 4-day non-brushing randomized controlled clinical trial.Journal of periodontology · 2026Trial
- A new and eco-friendly disinfectant for antimicrobial-resistant bacteria: ozone nano water.BMC microbiology · 2026Article
- Electrolyzed saline coolant mitigates bacterial airborne deposition in dental procedures.Frontiers in oral health · 2026Article
- Preparation of ruthenium electrode materials and their application to the bactericidal properties of acidic electrolyzed oxidizing water.RSC advances · 2025Article
- Thymol combined with SAEW for the eradication of matureFrontiers in microbiology · 2025Article
- Research Progress on Bacteria-Reducing Pretreatment Technology of Meat.Foods (Basel, Switzerland) · 2024Review
- Antibacterial action of slightly acidic electrolytic water againstFrontiers in microbiology · 2024Article
- Bactericidal Efficacy and Mechanisms of Non-Electrolytic Slightly Acidic Hypochlorous Water onFoods (Basel, Switzerland) · 2023Article
- Comparison of the efficacy of physical and chemical strategies for the inactivation of biofilm cells of foodborne pathogens.Food science and biotechnology · 2023Review
- Electrolyzed Water and Its Pharmacological Activities: A Mini-Review.Molecules (Basel, Switzerland) · 2022Review
- Variation of Soil Microbial Community and Sterilization toFrontiers in microbiology · 2022Article
- Acidic Electrolyzed Water Inhibits the Viability ofFrontiers in medicine · 2022Article
- Inactivation and Membrane Damage Mechanism of Slightly Acidic Electrolyzed Water onMolecules (Basel, Switzerland) · 2021Article
- Electrolyzed Oxidizing Water and Its Applications as Sanitation and Cleaning Agent.Food engineering reviews · 2021Review
- Article
- Oxidation-reduction potential as a new marker for oxidative stress: Correlation to male infertility.Investigative and clinical urology · 2017Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Foodborne disease outbreak caused by food microbiological contamination is a serious public health problem. Slightly acidic electrolyzed water (SAEW), a new ultra-high effect and wide-spectrum disinfectant that is colourless, odourless, and harmless to humans and the environment, is directly used on food surfaces in Japan and America. However, the underlying inactivation mechanism remains unknown. In this study, biochemical and cellular changes were observed to investigate the bactericidal mechanism of SAEW against Escherichia coli (E. coli). The results indicated that SAEW with a pH of 6.40, an oxidation-reduction potential (ORP) of 910 mV, an available chlorine concentration (ACC) of 60 mg/L, and a volume ratio of 20:1, produced the most effective sterilization action. A fluorescence-based live-dead assay was further used to demonstrate the sterilized effect and the cell esterase activity damage caused by SAEW. During the observation period, within 10 min, the cell morphology changed, which was characterized by cell expansion, cell elongation and increased membrane permeability. Meanwhile, reactive oxygen substances (ROS) were released in the bacterial cells. E. coli inactivation and apoptosis induced by SAEW were observed. Our findings illustrate that the bactericidal effects of SAEW against E. coli occurred through cellular and biochemical mechanisms of cell necrosis and apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.