ArticleScientific reports2017
Legionella pneumophila infection activates bystander cells differentially by bacterial and host cell vesicles.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 37 citations in OpenAlex.
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- Lipid A in outer membrane vesicles shields bacteria from polymyxins.Journal of extracellular vesicles · 2024Article
- Extracellular Vesicles and Their Role in Lung Infections.International journal of molecular sciences · 2023Review
- Surface proteome of plasma extracellular vesicles as mechanistic and clinical biomarkers for malaria.Infection · 2023Article
- Translocated Legionella pneumophila small RNAs mimic eukaryotic microRNAs targeting the host immune response.Nature communications · 2022Article
- Extracellular Vesicles: Recent Insights Into the Interaction Between Host and Pathogenic Bacteria.Frontiers in immunology · 2022Review
- The Role of Extracellular Vesicles from Human Macrophages on Host-Pathogen Interaction.International journal of molecular sciences · 2021Review
- Bacterial Membrane Vesicles in Pneumonia: From Mediators of Virulence to Innovative Vaccine Candidates.International journal of molecular sciences · 2021Review
- CF monocyte-derived macrophages have an attenuated response to extracellular vesicles secreted by airway epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2021Article
- Unlocking the Power of Exosomes for Crossing Biological Barriers in Drug Delivery.Pharmaceutics · 2021Review
- Detection and segmentation of morphologically complex eukaryotic cells in fluorescence microscopy images via feature pyramid fusion.PLoS computational biology · 2020Article
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Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles from eukaryotic cells and outer membrane vesicles (OMVs) released from gram-negative bacteria have been described as mediators of pathogen-host interaction and intercellular communication. Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. The differential effect of bacterial and host cell vesicles in L. pneumophila infection is unknown so far. We infected THP-1-derived or primary human macrophages with L. pneumophila and isolated supernatant vesicles by differential centrifugation. We observed an increase of exosomes in the 100 k pellet by nanoparticle tracking analysis, electron microscopy, and protein markers. This fraction additionally contained Legionella LPS, indicating also the presence of OMVs. In contrast, vesicles in the 16 k pellet, representing microparticles, decreased during infection. The 100 k vesicle fraction activated uninfected primary human alveolar epithelial cells, A549 cells, and THP-1 cells. Epithelial cell activation was reduced by exosome depletion (anti-CD63, or GW4869), or blocking of IL-1β in the supernatant. In contrast, the response of THP-1 cells to vesicles was reduced by a TLR2-neutralizing antibody, UV-inactivation of bacteria, or - partially - RNase-treatment of vesicles. Taken together, we found that during L. pneumophila infection, neighbouring epithelial cells were predominantly activated by exosomes and cytokines, whereas myeloid cells were activated by bacterial OMVs.
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