ArticleScientific reports2017
Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 96 citations in OpenAlex.
- Monosodium glutamate protects DNA of freeze-dried somatic cells and improves development of somatic cell nuclear transfer embryos.The Journal of reproduction and development · 2026Article
- Synergistic enhancement of industrial adaptability in Lactiplantibacillus plantarum BF_15 through glutathione-mediated redox and DNA repair pathways.World journal of microbiology & biotechnology · 2026Article
- Narrative review of strategies for blood product shelf-life extension.Transfusion · 2026Review
- Principles Underlying Cryopreservation and Freeze-Drying of Cells.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Lyophilization of Adeno-Associated Virus Serotypes for Storage and Global Distribution.Biomedicines · 2025Article
- Immunogenicity and safety in animals of a lyophilized live attenuated hepatitis A vaccine following stabilizer optimization.Scientific reports · 2025Article
- Interactions of Sucrose and Trehalose with Lysozyme in Different Media: A Perspective from Atomistic Molecular Dynamics Simulations.Molecular pharmaceutics · 2025Article
- Ministring DNA (msDNA): a novel linear covalently-closed DNA with enhanced stability for gene and cell therapy applications.Scientific reports · 2025Article
- A vacuum lyophilization and bacterial tablet-based method for culture medium evaluation and potential use in probiotic products.Frontiers in microbiology · 2025Article
- Engineering a Microfluidic Platform to Cryopreserve Stem Cells: A DMSO-Free Sustainable Approach.Advanced healthcare materials · 2024Article
- Characterization and Validation of a Lyophilized Loop-Mediated Isothermal Amplification Method for the Detection of Esox lucius.Applied biochemistry and biotechnology · 2024Article
- Artificial cells for in vivo biomedical applications through red blood cell biomimicry.Nature communications · 2024Review
- Heat Flux Analysis and Assessment of Drying Kinetics during Lyophilization of Fruits in a Pilot-Scale Freeze Dryer.Foods (Basel, Switzerland) · 2023Article
- Lyophilization for Formulation Optimization of Drug-Loaded Thermoresponsive Polyelectrolyte Complex Nanogels from Functionalized Hyaluronic Acid.Pharmaceutics · 2023Article
- Article
- Vacuum Foam Drying Method Improved the Thermal Stability and Long-Term Shelf Life of a Live Attenuated Newcastle Disease Virus Vaccine.AAPS PharmSciTech · 2022Article
- Combined optical fluorescence microscopy and X-ray tomography reveals substructures in cell nuclei in 3D.Biomedical optics express · 2022Article
- Long-term and short-term preservation strategies for tissue engineering and regenerative medicine products: state of the art and emerging trends.PNAS nexus · 2022Review
- Developing whole cell standards for the microbiome field.Microbiome · 2022Article
- Water Dynamics in Cancer Cells: Lessons from Quasielastic Neutron Scattering.Medicina (Kaunas, Lithuania) · 2022Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose alone or in a mixture of albumin and trehalose. Albumin was added to increase the glass transition temperature and storage stability. No viable cells were recovered after freeze-drying and rehydration. FTIR studies showed that membrane phase behavior of freeze-dried cells resembles that of fresh cells. However, one day after rehydration membrane phase separation was observed, irrespective of the presence or absence of trehalose during freeze-drying. Freeze-drying did not affect the overall protein secondary structure. Analysis of DNA damage via single cell gel electrophoresis ('comet assay') showed that DNA damage progressively increased with storage duration and temperature. DNA damage was prevented during storage at 4 °C. It is shown that trehalose reduces DNA damage during storage, whereas addition of albumin did not seem to have an additional protective effect on storage stability (i.e. DNA integrity) despite the fact that albumin increased the glass transition temperature. Taken together, DNA in freeze-dried somatic cells can be preserved using trehalose as protectant and storage at or below 4 °C.
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