ArticleProceedings of the National Academy of Sciences of the United States of America2017
Effect of ATP and regulatory light-chain phosphorylation on the polymerization of mammalian nonmuscle myosin II.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Role of the nonhelical tailpiece of myosin-II in regulating filament architecture and function.The Journal of cell biology · 2026Article
- A song of heads and tails: myosin II conformational regulation and filament dynamics shape force generation in non-muscle cells.Biophysical reviews · 2026Review
- Multiscale modelling shows how cell-ECM interactions impact ECM fibre alignment and cell detachment.PLoS computational biology · 2025Article
- Structure, regulation, and mechanisms of nonmuscle myosin-2.Cellular and molecular life sciences : CMLS · 2024Review
- Local monomer levels and established filaments potentiate non-muscle myosin 2 assembly.The Journal of cell biology · 2024Article
- SuggestingCells · 2024Article
- Exploring the nexus between MYH9 and tumors: novel insights and new therapeutic opportunities.Frontiers in cell and developmental biology · 2024Review
- Article
- Non-muscle myosin 2 at a glance.Journal of cell science · 2023Review
- Thermo-reversible gelation of myofibrillar protein: Relationship between coiled-coil and thermal reversibility.Current research in food science · 2023Article
- Synaptopodin stress fiber and contractomere at the epithelial junction.The Journal of cell biology · 2022Article
- Filament evanescence of myosin II and smooth muscle function.The Journal of general physiology · 2021Review
- Article
- Non-Muscle Myosin II in Axonal Cell Biology: From the Growth Cone to the Axon Initial Segment.Cells · 2020Review
- Effect of allosteric inhibition of non-muscle myosin 2 on its intracellular diffusion.Scientific reports · 2020Article
- A novel MYH14 mutation in a Chinese family with autosomal dominant nonsyndromic hearing loss.BMC medical genetics · 2020Article
- Muscle myosins form folded monomers, dimers, and tetramers during filament polymerization in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Linking the Landscape ofCells · 2020Review
- The central role of the tail in switching off 10S myosin II activity.The Journal of general physiology · 2019Article
- Anchoring cortical granules in the cortex ensures trafficking to the plasma membrane for post-fertilization exocytosis.Nature communications · 2019Article
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Authors and funding
7 authors.
Funding
Abstract
Addition of 1 mM ATP substantially reduces the light scattering of solutions of polymerized unphosphorylated nonmuscle myosin IIs (NM2s), and this is reversed by phosphorylation of the regulatory light chain (RLC). It has been proposed that these changes result from substantial depolymerization of unphosphorylated NM2 filaments to monomers upon addition of ATP, and filament repolymerization upon RLC-phosphorylation. We now show that the differences in myosin monomer concentration of RLC-unphosphorylated and -phosphorylated recombinant mammalian NM2A, NM2B, and NM2C polymerized in the presence of ATP are much too small to explain their substantial differences in light scattering. Rather, we find that the decrease in light scattering upon addition of ATP to polymerized unphosphorylated NM2s correlates with the formation of dimers, tetramers, and hexamers, in addition to monomers, an increase in length, and decrease in width of the bare zones of RLC-unphosphorylated filaments. Both effects of ATP addition are reversed by phosphorylation of the RLC. Our data also suggest that, contrary to previous models, assembly of RLC-phosphorylated NM2s at physiological ionic strength proceeds from folded monomers to folded antiparallel dimers, tetramers, and hexamers that unfold and polymerize into antiparallel filaments. This model could explain the dynamic relocalization of NM2 filaments in vivo by dephosphorylation of RLC-phosphorylated filaments, disassembly of the dephosphorylated filaments to folded monomers, dimers, and small oligomers, followed by diffusion of these species, and reassembly of filaments at the new location following rephosphorylation of the RLC.
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