ArticleBMC microbiology2017
Improved detection of genus-specific Alphavirus using a generic TaqMan® assay.
Article in BMC microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Sentinel surveillance of acute febrile illness in a Brazilian Amazon region: laboratory findings and epidemiological implications.Revista do Instituto de Medicina Tropical de Sao Paulo · 2026Article
- Metagenomic detection of central nervous system infections missedby conventional testing.JCI insight · 2025Article
- Pathogenesis and clinical management of arboviral diseases.World journal of virology · 2025Review
- Excellent capability for molecular detection ofJournal of clinical microbiology · 2025Article
- Detection of coinfection with Primate Erythroparvovirus 1 and arboviruses (DENV, CHIKV and ZIKV) in individuals with acute febrile illness in the state of Rio Grande do Norte in 2016.PLoS neglected tropical diseases · 2023Article
- Article
- Understanding the Biology and Immune Pathogenesis of Chikungunya Virus Infection for Diagnostic and Vaccine Development.Viruses · 2022Review
- Recent molecular techniques for the diagnosis of Zika and Chikungunya infections: A systematic review.Heliyon · 2022Article
- Everglades virus evolution: Genome sequence analysis of the envelope 1 protein reveals recent mutation and divergence in South Florida wetlands.Virus evolution · 2022Article
- Developing a direct acting, orally available antiviral agent in a pandemic: the evolution of molnupiravir as a potential treatment for COVID-19.Current opinion in virology · 2021Review
- Invasive Burmese pythons alter host use and virus infection in the vector of a zoonotic virus.Communications biology · 2021Article
- Plasma virome of 781 Brazilians with unexplained symptoms of arbovirus infection include a novel parvovirus and densovirus.PloS one · 2020Article
- Smartphone-Based Mobile Detection Platform for Molecular Diagnostics and Spatiotemporal Disease Mapping.Analytical chemistry · 2018Article
- Confronting the Emerging Threat to Public Health in Northern Australia of Neglected Indigenous Arboviruses.Tropical medicine and infectious disease · 2017Article
- Neglected Australian Arboviruses and Undifferentiated Febrile Illness: Addressing Public Health Challenges Arising From the 'Developing Northern Australia' Government Policy.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlphaviruses are arthropod borne RNA viruses of medical importance. Geographical expansion of mosquitoes of the Aedes genus in the past decades has been associated with major Alphavirus-associated outbreaks. Climate changes and intensification of air travels have favored vector expansion and virus dissemination in new territories leading to virus emergence not only in tropical areas but also in temperate regions. The detection of emergence is based upon surveillance networks with epidemiological and laboratory investigation.
methodA specific, sensitive and rapid screening test for genus-specific Alphavirus is critically required. To address this issue, we developed a new molecular assay targeting nsP4 gene and using a TaqMan® real time RT-PCR method for the specific detection of all major Alphavirus genus members.
resultsThis assay was tested for specificity using several Alphavirus species. We also tested successfully clinical sensitivity using patient's samples collected during the Chikungunya outbreak of 2005-2006 in the Indian Ocean.
conclusionsThis new pan-Alphavirus molecular diagnostic tool offers great potential for exclusion diagnosis and emergence detection given its broad specificity restricted to Alphavirus genus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.