ArticleBMC genomics2017
Histological and transcriptomic effects of 17α-methyltestosterone on zebrafish gonad development.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 85 citations in OpenAlex.
- Dietary pine pollen induces masculinization in Nile tilapia (Oreochromis niloticus, L. 1758) by modulating sex-biased gene expression and steroid hormone profiles.Biology of sex differences · 2026Article
- Resistance of AdultIntegrative organismal biology (Oxford, England) · 2026Article
- Impact of fluoride on thyroid function and histopathology in cyprinus carpio: Implications for aquatic ecosystems.Toxicology reports · 2025Article
- Integrated lncRNA and mRNA Transcriptome Analyses of IGF1 and IGF2 Stimulated Ovaries Reveal Genes and Pathways Potentially Associated with Ovarian Development and Oocyte Maturation in Golden Pompano (Animals : an open access journal from MDPI · 2025Article
- Germ cell progression through zebrafish spermatogenesis declines with age.Development (Cambridge, England) · 2024Article
- Article
- Steroid hormone-deprived sex reversal in cyp11a1 mutant XX tilapia experiences an ovary-like stage at molecular level.Communications biology · 2024Article
- Molecular and Physiological Effects of 17α-methyltestosterone on Sex Differentiation of Black Rockfish,Genes · 2024Article
- Effects of exogenous steroid hormones on growth, body color, and gonadal development in the Opsariichthys bidens.Fish physiology and biochemistry · 2024Article
- Identification of Insulin-like Growth Factor (IGF) Family Genes in the Golden Pompano,International journal of molecular sciences · 2024Article
- Exploring the Effects of Rearing Densities on Epigenetic Modifications in the Zebrafish Gonads.International journal of molecular sciences · 2023Article
- Comparative Analysis of miRNA-mRNA Regulation in the Testes ofInternational journal of molecular sciences · 2023Article
- A Simple Method for Inducing Masculinization of Zebrafish Stocks Using 17α-Methyltestosterone.Zebrafish · 2022Review
- Environmentally-induced sex reversal in fish with chromosomal vs. polygenic sex determination.Environmental research · 2022Article
- High Polymorphism in theAnimals : an open access journal from MDPI · 2022Article
- Transcriptomes of testis and pituitary from male Nile tilapia (O. niloticus L.) in the context of social status.PloS one · 2022Article
- Epigenetic Regulation of Phenotypic Sexual Plasticity Inducing Skewed Sex Ratio in Zebrafish.Frontiers in cell and developmental biology · 2022Article
- Transcriptome analysis of five ovarian stages reveals gonad maturation in female Macrobrachium nipponense.BMC genomics · 2021Article
- Differential expression analysis and identification of sex-related genes by gonad transcriptome sequencing in estradiol-treated and non-treated Ussuri catfish Pseudobagrus ussuriensis.Fish physiology and biochemistry · 2021Article
- Integrated Analysis of mRNA- and miRNA-Seq in the Ovary of Rare MinnowFrontiers in genetics · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSex hormones play important roles in teleost ovarian and testicular development. In zebrafish, ovarian differentiation appears to be dictated by an oocyte-derived signal via Cyp19a1a aromatase-mediated estrogen production. Androgens and aromatase inhibitors can induce female-to-male sex reversal, however, the mechanisms underlying gonadal masculinisation are poorly understood. We used histological analyses together with RNA sequencing to characterise zebrafish gonadal transcriptomes and investigate the effects of 17α-methyltestosterone on gonadal differentiation.
resultsAt a morphological level, 17α-methyltestosterone (MT) masculinised gonads and accelerated spermatogenesis, and these changes were paralleled in masculinisation and de-feminisation of gonadal transcriptomes. MT treatment upregulated expression of genes involved in male sex determination and differentiation (amh, dmrt1, gsdf and wt1a) and those involved in 11-oxygenated androgen production (cyp11c1 and hsd11b2). It also repressed expression of ovarian development and folliculogenesis genes (bmp15, gdf9, figla, zp2.1 and zp3b). Furthermore, MT treatment altered epigenetic modification of histones in zebrafish gonads. Contrary to expectations, higher levels of cyp19a1a or foxl2 expression in control ovaries compared to MT-treated testes and control testes were not statistically significant during early gonad development (40 dpf).
conclusionOur study suggests that both androgen production and aromatase inhibition are important for androgen-induced gonadal masculinisation and natural testicular differentiation in zebrafish.
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