Evidence map›Paper›PMID 28738722›Full record

ArticlePharmaceutical biology2017

Effects of indirubin and isatin on cell viability, mutagenicity, genotoxicity and BAX/ERCC1 gene expression.

Manoela Viar Fogaça, Priscila de Matos Cândido-Bacani, Lucas Milanez Benicio, Lara Martinelli Zapata, Priscilla de Freitas Cardoso, Marcelo Tempesta de Oliveira, Tamara Regina Calvo, Eliana Aparecida Varanda, Wagner Vilegas, Ilce Mara de Syllos Cólus

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmaceutical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. ProspectingJournal of enzyme inhibition and medicinal chemistry · 2023
    Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Manoela Viar Fogaçaa Department of General Biology, Center of Biological Sciences , State University of Londrina , Londrina , Brazil.
Priscila de Matos Cândido-Bacania Department of General Biology, Center of Biological Sciences , State University of Londrina , Londrina , Brazil.
Lucas Milanez Benicioa Department of General Biology, Center of Biological Sciences , State University of Londrina , Londrina , Brazil.
Lara Martinelli Zapataa Department of General Biology, Center of Biological Sciences , State University of Londrina , Londrina , Brazil.
Priscilla de Freitas Cardosoa Department of General Biology, Center of Biological Sciences , State University of Londrina , Londrina , Brazil.
Marcelo Tempesta de Oliveiraa Department of General Biology, Center of Biological Sciences , State University of Londrina , Londrina , Brazil.
Tamara Regina Calvob Araraquara Institute of Chemistry , São Paulo State University , Araraquara , Brazil.
Eliana Aparecida Varandac Araraquara Faculty of Pharmaceutical Sciences, Department of Biological Sciences , São Paulo State University , Araraquara , Brazil.
Wagner Vilegasb Araraquara Institute of Chemistry , São Paulo State University , Araraquara , Brazil.
Ilce Mara de Syllos Cólusa Department of General Biology, Center of Biological Sciences , State University of Londrina , Londrina , Brazil.
Universidade Estadual de Londrina · BRCentro Universitário de Araraquara · BRUniversidade Estadual Paulista (Unesp) · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

contextIndigofera suffruticosa Miller (Fabaceae) and I. truxillensis Kunth produce compounds, such as isatin (ISA) and indirubin (IRN), which possess antitumour properties. Their effects in mammalian cells are still not very well understood.

objectiveWe evaluated the activities of ISA and/or IRN on cell viability and apoptosis in vitro, their genotoxic potentials in vitro and in vivo, and the IRN- and ISA-induced expression of ERCC1 or BAX genes. MATERIALS AND

methodsHeLa and/or CHO-K1 cell lines were tested (3 or 24 h) in the MTT, Trypan blue exclusion, acridine orange/ethidium bromide, cytokinesis-blocked micronucleus (CBMN) and comet (36, 24 and 72 h) tests after treatment with IRN (0.1 to 200 μM) or ISA (0.5 to 50 μM). Gene expression was measured by RT-qPCR in HeLa cells. Swiss albino mice received IRN (3, 4 or 24 h) by gavage (50, 100 and 150 mg/kg determined from the LD

resultsIRN reduced the viability of CHO-K1 (24 h; 5 to 200 μM) and HeLa cells (10 to 200 μM), and was antiproliferative in the CBMN test (CHO-K1: 0.5 to 10 μM; HeLa: 5 and 10 μM). The drug did not induce apoptosis, micronucleus neither altered gene expression. IRN and ISA were genotoxic for HeLa cells (3 and 24 h) at all doses tested. IRN (100 and 150 mg/kg) also induced genotoxicity in vivo (4 h).

conclusionIRN and ISA have properties that make them candidates as chemotherapeutics for further pharmacological investigations.

Indexed as

AnimalsAntibiotics, Antineoplasticbcl-2-Associated X ProteinCell SurvivalCHO CellsCricetinaeCricetulusDNA-Binding ProteinsDNA DamageDose-Response Relationship, DrugEndonucleasesFemaleGene ExpressionHeLa CellsHumansIndolesAntibiotics, AntineoplasticBAX protein, humanbcl-2-Associated X ProteinDNA-Binding ProteinsEndonucleasesERCC1 protein, humanindirubinIndolesIsatinPlant ExtractsapoptosisCHO-K1 cellscomet assaycytokinesis-blocked micronucleus assayHeLa cellsIndigofera suffruticosaIndigofera truxillensis

Identifiers

PMID28738722
PMCPMC7011876
OpenAlexW2738524238

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.