Evidence map›Paper›PMID 28736753›Full record

ArticleHeliyon2017

Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks.

Isabel G Fernández de Mera, Ilias Chaligiannis, Angélica Hernández-Jarguín, Margarita Villar, Lourdes Mateos-Hernández, Anna Papa, Smaragda Sotiraki, Francisco Ruiz-Fons, Alejandro Cabezas-Cruz, Christian Gortázar and 1 more

Open access · goldAbstract read
In one paragraph

Article in Heliyon, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Proteomics ofInternational journal of nanomedicine · 2021
    Article
  6. Diagnostic Testing for Crimean-Congo Hemorrhagic Fever.Journal of clinical microbiology · 2020
    Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 5 countries.

Isabel G Fernández de MeraSaBio. Instituto de Investigación de Recursos Cinegéticos, IREC-CSIC-UCLM-JCCM, 13005 Ciudad Real, Spain.
Ilias ChaligiannisDepartment of Microbiology, Medical School, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.
Angélica Hernández-JarguínSaBio. Instituto de Investigación de Recursos Cinegéticos, IREC-CSIC-UCLM-JCCM, 13005 Ciudad Real, Spain.
Margarita VillarSaBio. Instituto de Investigación de Recursos Cinegéticos, IREC-CSIC-UCLM-JCCM, 13005 Ciudad Real, Spain.
Lourdes Mateos-HernándezSaBio. Instituto de Investigación de Recursos Cinegéticos, IREC-CSIC-UCLM-JCCM, 13005 Ciudad Real, Spain.
Anna PapaDepartment of Microbiology, Medical School, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.
Smaragda SotirakiVeterinary Research Institute, Hellenic Agricultural Organisation-Demeter, NAGREF Campus, 57001 Thermi, Thessaloniki, Greece.
Francisco Ruiz-FonsSaBio. Instituto de Investigación de Recursos Cinegéticos, IREC-CSIC-UCLM-JCCM, 13005 Ciudad Real, Spain.
Alejandro Cabezas-CruzInstitute of Parasitology, Biology Center of the Academy of Sciences of the Czech Republic, 37005 České Budějovice, Czech Republic.
Christian GortázarSaBio. Instituto de Investigación de Recursos Cinegéticos, IREC-CSIC-UCLM-JCCM, 13005 Ciudad Real, Spain.
José de la FuenteSaBio. Instituto de Investigación de Recursos Cinegéticos, IREC-CSIC-UCLM-JCCM, 13005 Ciudad Real, Spain.
Consejo Superior de Investigaciones Científicas · ESAristotle University of Thessaloniki · GRInstituto de Investigación en Recursos Cinegéticos · ESNational Agricultural Research Foundation · GROklahoma State University · USUniversity Hospital of Larissa · GRUniversity of South Bohemia in České Budějovice · CZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne zoonotic disease caused by the CCHF virus (CCHFV). In this study, an experimental approach combining RT-PCR and proteomics was used for the identification and characterization of CCHFV in 106 ticks from 7 species that were collected from small ruminants in Greece. The methodological approach included an initial screening for CCHFV by RT-PCR followed by proteomics analysis of positive and control negative tick samples. This novel approach allowed the identification of CCHFV-positive ticks and provided additional information to corroborate the RT-PCR findings using a different approach. Two ticks,

Indexed as

EvolutionGeneticsInfectious diseasePublic healthVeterinary scienceVirology

Identifiers

PMID28736753
PMCPMC5508474
OpenAlexW2735199570

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.