ArticleBiomedical optics express2017
High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT).
Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Optimizing immunofluorescence with high-dynamic-range imaging to enhance PD-L1 expression evaluation for 3D pathology assessment from NSCLC tumor tissue.Scientific reports · 2024Article
- Quantitative analysis of vascular changes during photoimmunotherapy using speckle variance optical coherence tomography (SV-OCT).Biomedical optics express · 2021Article
- Article
- Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes.Journal of biomedical optics · 2019Article
- Comparison of time- and angular-domain scatter rejection in mesoscopic optical projection tomography: a simulation study.Biomedical optics express · 2019Article
- 3D mesoscopic fluorescence tomography for imaging micro-distribution of antibody-photon absorber conjugates during near infrared photoimmunotherapy in vivo.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Imaging stem cell distribution, growth, migration, and differentiation in 3-D scaffolds for bone tissue engineering using mesoscopic fluorescence tomography.Biotechnology and bioengineering · 2018Article
- Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan.Scientific reports · 2017Article
- High dynamic range fluorescence imaging.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
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Authors and funding
8 authors.
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Abstract
Three-dimensional fluorescence laminar optical tomography (FLOT) can achieve resolutions of 100-200 µm and penetration depths of 2-3 mm. FLOT has been used in tissue engineering, neuroscience, as well as oncology. The limited dynamic range of the charge-coupled device-based system makes it difficult to image fluorescent samples with a large concentration difference, limits its penetration depth, and diminishes the quantitative accuracy of 3D reconstruction data. Here, incorporating the high-dynamic-range (HDR) method widely used in digital cameras, we present HDR-FLOT, increasing penetration depth and improving the ability to image fluorescent samples with a large concentration difference. The method was tested using an agar phantom and a B6 mouse for brain imaging
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