ArticlePlant methods2017
Elucidating the role of highly homologous
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- The StUBC18-StPUB40 pair negatively regulate drought stress tolerance and influences tuber yield in potato.Horticulture research · 2025Article
- Genome-wide identification of wheat Ubiquitin-conjugating enzyme gene family and its role in wheat powdery mildew disease resistance.BMC plant biology · 2025Article
- Plant ubiquitin E2 enzymes UBC32, UBC33, and UBC34 are involved in ERAD and function in host stress tolerance.BMC plant biology · 2025Article
- StUBC13, a Ubiquitin-Conjugating Enzyme, Positively Regulates Salt and Osmotic Stresses in Potato.International journal of molecular sciences · 2024Article
- A simple and efficient gene functional analysis method for studying the growth and development of peach seedlings.Horticulture research · 2024Article
- Multiple factors and features dictate the selective production of ct-siRNA in Arabidopsis.Communications biology · 2024Article
- Genomic Insights into High-Altitude Adaptation: A Comparative Analysis ofInternational journal of molecular sciences · 2024Article
- Argonaute 5 family proteins play crucial roles in the defence against Cymbidium mosaic virus and Odontoglossum ringspot virus in Phalaenopsis aphrodite subsp. formosana.Molecular plant pathology · 2021Article
- A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants.Frontiers in plant science · 2021Review
- The Ubiquitin Conjugating Enzyme: An Important Ubiquitin Transfer Platform in Ubiquitin-Proteasome System.International journal of molecular sciences · 2020Review
- Perturbations of the ZED1 pseudokinase activate plant immunity.PLoS pathogens · 2019Article
- Comparative Proteomic Analysis Reveals Elevated Capacity for Photosynthesis in Polyphenol Oxidase Expression-SilencedInternational journal of molecular sciences · 2018Article
- The Tomato U-Box Type E3 Ligase PUB13 Acts With Group III Ubiquitin E2 Enzymes to Modulate FLS2-Mediated Immune Signaling.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVirus-induced gene silencing (VIGS) has been used in many plant species as an attractive post transcriptional gene silencing (PTGS) method for studying gene function either individually or at large-scale in a high-throughput manner. However, the specificity and efficiency for knocking down members of a highly homologous gene family have remained to date a significant challenge in VIGS due to silencing of off-targets.
resultsHere we present an improved method for the selection and evaluation of gene fragments used for VIGS to specifically and efficiently knock down members of a highly homologous gene family. Using this method, we knocked down twelve and four members, respectively of group III of the gene family encoding ubiquitin-conjugating enzymes (E2) in
conclusionsWe have found that employment of a gene fragment as short as approximately 70 base pairs (bp) that contains at least three mismatched nucleotides to other genes within any 21-bp sequences prevents silencing of off-target(s) in VIGS. This improved approach in the selection and evaluation of gene fragments allows for specific and efficient knocking down of highly homologous members of a gene family. Using this approach, we implicated
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.