ArticleEuropean journal of histochemistry : EJH2017
Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation.
Article in European journal of histochemistry : EJH, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 4 citations in OpenAlex.
- Heterotopic ossification in a patient with diffuse idiopathic skeletal hyperostosis: Input from histological findings.European journal of histochemistry : EJH · 2020Article
- Bu-M-P-ing Iron: How BMP Signaling Regulates Muscle Growth and Regeneration.Journal of developmental biology · 2020Review
- Analysis of mineral apposition rates during alveolar bone regeneration over three weeks following transfer of BMP-2/7 gene via in vivo electroporationEuropean journal of histochemistry : EJH · 2018Article
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Authors and funding
16 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous in vivo electroporation. We applied this method to transfer the bone morphogenetic protein (BMP) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after BMP gene transfer by in vivo electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood. In the present study, we transferred the BMP gene into rat skeletal muscles. We then stained tissues for several muscle-derived stem cell markers (e.g., Pax7, M-cadherin), muscle regeneration-related markers (e.g., Myod1, myogenin), and an inflammatory cell marker (CD68) to follow cell differentiation over time. Our results indicate that, in the absence of BMP, the cell population undergoes muscle regeneration, whereas in its presence, it can differentiate into osteogenic cells. Commitment towards either muscle regeneration or induction of ectopic bone formation appears to occur five to seven days after BMP gene transfer.
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