ArticleBiophysical journal2017
Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed, 73 citations in OpenAlex.
- The Many Layers of Membrane Biophysics: Environment-Sensitive Fluorophores Report on Structural Organization of Biological Membranes at Various Depths.Analytical chemistry · 2026Article
- Protocol for microfluidic-based high-precision general polarization fluorescence microscopy of lipid packing in membrane vesicles.STAR protocols · 2026Article
- Disorder in Order-Related Membrane Biophysical Parameters: An In-Depth Analysis of Di-4-ANEPPDHQ Generalized Polarization.The journal of physical chemistry letters · 2026Article
- VISION - an open-source software for automated multi-dimensional image analysis of cellular biophysics.Journal of cell science · 2024Article
- Tensing Flipper: Photosensitized Manipulation of Membrane Tension, Lipid Phase Separation, and Raft Protein Sorting in Biological Membranes.Journal of the American Chemical Society · 2024Article
- Modulating glycosphingolipid metabolism and autophagy improves outcomes in pre-clinical models of myeloma bone disease.Nature communications · 2022Article
- Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia.International journal of molecular sciences · 2022Article
- Understanding immune signaling using advanced imaging techniques.Biochemical Society transactions · 2022Review
- Long-term STED imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes.Biophysical reports · 2021Article
- Investigation of the Membrane Fluidity Regulation of Fatty Acid Intracellular Distribution by Fluorescence Lifetime Imaging of Novel Polarity Sensitive Fluorescent Derivatives.International journal of molecular sciences · 2021Article
- Article
- What Does Time-Dependent Fluorescence Shift (TDFS) in Biomembranes (and Proteins) Report on?Frontiers in chemistry · 2021Review
- Super-resolution RESOLFT microscopy of lipid bilayers using a fluorophore-switch dyad.Chemical science · 2020Article
- z-STED Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and Dynamics.Biophysical journal · 2020Article
- Mechanical Tension of Biomembranes Can Be Measured by Super Resolution (STED) Microscopy of Force-Induced Nanotubes.Nano letters · 2020Article
- Patches and Blebs: A Comparative Study of the Composition and Biophysical Properties of Two Plasma Membrane Preparations from CHO Cells.International journal of molecular sciences · 2020Article
- Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells.Frontiers in immunology · 2020Review
- Membrane Heterogeneity Beyond the Plasma Membrane.Frontiers in cell and developmental biology · 2020Review
- Fluidity and Lipid Composition of Membranes of Peroxisomes, Mitochondria and the ER From Oleic Acid-InducedFrontiers in cell and developmental biology · 2020Article
- Fluorescent Protein-Based Indicators for Functional Super-Resolution Imaging of Biomolecular Activities in Living Cells.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The lateral organization of molecules in the cellular plasma membrane plays an important role in cellular signaling. A critical parameter for membrane molecular organization is how the membrane lipids are packed. Polarity-sensitive dyes are powerful tools to characterize such lipid membrane order, employing, for example, confocal and two-photon microscopy. The investigation of potential nanodomains, however, requires the use of superresolution microscopy. Here, we test the performance of the polarity-sensitive membrane dyes Di-4-ANEPPDHQ, Di-4-AN(F)EPPTEA, and NR12S in superresolution stimulated emission depletion microscopy. Measurements on cell-derived membrane vesicles, in the plasma membrane of live cells, and on single virus particles, show the high potential of these dyes for probing nanoscale membrane heterogeneity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.