ArticleActa veterinaria Scandinavica2017
In vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens.
Article in Acta veterinaria Scandinavica, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 84 citations in OpenAlex.
- Nanotechnology-Based Immunostimulants in Aquaculture: Mechanisms, Advances and Practical Applications.Veterinary medicine and science · 2026Review
- Silver and Zinc Oxide Nanoparticles for Effective Aquaculture Wastewater Treatment.Nanomaterials (Basel, Switzerland) · 2025Article
- Silver Nanoparticles as Antimicrobial Agents in Veterinary Medicine: Current Applications and Future Perspectives.Nanomaterials (Basel, Switzerland) · 2025Review
- Dietary supplementation with nano-zinc modulates the expression of the antiviral immune geneFrontiers in immunology · 2025Article
- Biocontrol of multidrug resistant pathogens isolated from fish farms using silver nanoparticles combined with hydrogen peroxide insight to its modulatory effect.Scientific reports · 2024Article
- Biogenic synthesis of silver nanoparticle byFrontiers in microbiology · 2024Article
- The Antimicrobial Applications of Nanoparticles in Veterinary Medicine: A Comprehensive Review.Antibiotics (Basel, Switzerland) · 2023Review
- Beneficial and toxicological aspects of zinc oxide nanoparticles in animals.Veterinary medicine and science · 2022Review
- Therapeutic Intervention with Dietary Chitosan Nanoparticles Alleviates Fish Pathological and Molecular Systemic Inflammatory Responses against Infections.Marine drugs · 2022Article
- Antimicrobial activity of chemically and biologically synthesized silver nanoparticles against some fish pathogens.Saudi journal of biological sciences · 2022Article
- Advantages and prospective challenges of nanotechnology applications in fish cultures: a comparative review.Environmental science and pollution research international · 2021Review
- Article
- Synergistic Effect of Biosynthesized Silver Nanoparticles and Natural Phenolic Compounds against Drug-Resistant Fish Pathogens and Their Cytotoxicity: An In Vitro Study.Marine drugs · 2021Article
- Overall assessment of antibiotic substitutes for pigs: a set of meta-analyses.Journal of animal science and biotechnology · 2021Article
- In vitro assessment of the antimicrobial efficacy of chitosan nanoparticles against major fish pathogens and their cytotoxicity to fish cell lines.Journal of fish diseases · 2020Article
- Metal Oxide Nanoparticles as Biomedical Materials.Biomimetics (Basel, Switzerland) · 2020Review
- Biocidal Activity of Metal Nanoparticles Synthesized byJournal of microbiology and biotechnology · 2020Article
- Genotoxicity of Silver Nanoparticles.Nanomaterials (Basel, Switzerland) · 2020Review
- The Role of Silver Nanoparticles in a Treatment Approach for Multidrug-ResistantInternational journal of nanomedicine · 2020Article
- Zinc Oxide nanoparticles induce oxidative and proteotoxic stress in ovarian cancer cells and trigger apoptosis Independent of p53-mutation status.Applied surface science · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundAntibiotic resistance is a global issue that threatens public health. The excessive use of antibiotics contributes to this problem as the genes of antibiotic resistance can be transferred between the bacteria in humans, animals and aquatic organisms. Metallic nanoparticles could serve as future substitutes for some conventional antibiotics because of their antimicrobial activity. The aim of this study was to evaluate the antimicrobial effects of silver and zinc oxide nanoparticles against major fish pathogens and assess their safety in vitro. Silver nanoparticles were synthesized by chemical reduction and characterized with UV-Vis spectroscopy, transmission electron microscopy and zeta sizer. The concentrations of silver and zinc oxide nanoparticles were measured using inductively coupled plasma-mass spectrometry. Subsequently, silver and zinc oxide nanoparticles were tested for their antimicrobial activity against Aeromonas hydrophila, Aeromonas salmonicida subsp. salmonicida, Edwardsiella ictaluri, Edwardsiella tarda, Francisella noatunensis subsp. orientalis, Yersinia ruckeri and Aphanomyces invadans and the minimum inhibitory concentrations were determined. MTT assay was performed on eel kidney cell line (EK-1) to determine the cell viability after incubation with nanoparticles. The interaction between silver nanoparticles and A. salmonicida was investigated by transmission electron microscopy.
resultsThe tested nanoparticles exhibited marked antimicrobial activity. Silver nanoparticles inhibited the growth of both A. salmonicida and A. invadans at a concentration of 17 µg/mL. Zinc oxide nanoparticles inhibited the growth of A. salmonicida, Y. ruckeri and A. invadans at concentrations of 15.75, 31.5 and 3.15 µg/mL respectively. Silver nanoparticles showed higher cell viability when compared to zinc oxide nanoparticles in the MTT assay. Transmission electron microscopy showed the attachment of silver nanoparticles to the bacterial membrane and disruption of its integrity.
conclusionsThis is the first study on inhibitory effects of silver and zinc oxide nanoparticles towards A. salmonicida and A. invadans. Moreover, zinc oxide nanoparticles inhibited the growth of Y. ruckeri. In low concentrations, silver nanoparticles were less cytotoxic than zinc oxide nanoparticles and represent an alternative antimicrobial compound against A. hydrophila, A. salmonicida and A. invadans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.