ArticleStem cell research2017
Generation of skeletal myogenic progenitors from human pluripotent stem cells using non-viral delivery of minicircle DNA.
Article in Stem cell research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Challenges and Considerations of Preclinical Development for iPSC-Based Myogenic Cell Therapy.Cells · 2024Review
- Multitasking muscle: engineering iPSC-derived myogenic progenitors to do more.Frontiers in cell and developmental biology · 2024Article
- Recapitulating human myogenesis ex vivo using human pluripotent stem cells.Experimental cell research · 2022Review
- Human muscle production in vitro from pluripotent stem cells: Basic and clinical applications.Seminars in cell & developmental biology · 2021Review
- Genomic Safe Harbor Expression of PAX7 for the Generation of Engraftable Myogenic Progenitors.Stem cell reports · 2021Article
- Improving therapeutic potential of non-viral minimized DNA vectors.Cell & gene therapy insights · 2020Article
- Global FKRP Registry: observations in more than 300 patients with Limb Girdle Muscular Dystrophy R9.Annals of clinical and translational neurology · 2020Observational
- Human muscle-derived CLEC14A-positive cells regenerate muscle independent of PAX7.Nature communications · 2019Article
- Cell therapy to improve regeneration of skeletal muscle injuries.Journal of cachexia, sarcopenia and muscle · 2019Review
- Hydrogel biomaterials and their therapeutic potential for muscle injuries and muscular dystrophies.Journal of the Royal Society, Interface · 2018Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Currently, the most efficient and promising approach for generating large numbers of engraftable human skeletal myogenic progenitors from pluripotent stem cells requires the conditional in vitro overexpression of PAX7 using lentiviral vectors. Because a non-integrating approach would be preferable to eliminate or minimize the risk associated with random genomic integration, here we investigate whether transient expression of PAX7 using minicircle DNA would enable the generation of functional pluripotent stem cell-derived myogenic progenitors, equivalent to those generated by lentivirus. Our results demonstrate that upon multiple transfections, the minicircle approach allows for the scalable generation of myogenic progenitors and these undergo efficient terminal differentiation in vitro. However, transplantation of minicircle-generated myogenic progenitors resulted in limited engraftment. This is probably due to less efficient delivery and more transient PAX7 expression in these cultures since PAX7 downregulation is accompanied by high level of spontaneous differentiation. Thus, although the in vitro data shows that the minicircle approach could potentially replace the use of lentivirus, improvements in the transfection/expression system will be necessary before it will be a feasible strategy for the generation of myogenic progenitors for cell replacement therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.